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OALib Journal期刊
ISSN: 2333-9721
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散斑壳菌对华山松凋落针叶的室内分解
DOI: 10.3969/j.issn.1000-2006.2014.03.015, PP. 77-82
Keywords: 散斑壳属真菌,华山松,凋落针叶,纤维素酶,微生物分解
Abstract:
为了探明散斑壳菌(lophodermiumspp.)分解利用针叶时的各纤维素酶酶解的作用机制和了解该菌的腐生性能,以华山松(pinusarmandii)新近凋落针叶为底物进行纯培养分解试验。试验以针叶为唯一碳源,采用试管培养液法,对lophodermiumconigenum、l.pinastri、l.kumaunicum、l.sichuanense和l.erlangshanense5种散斑壳菌进行纯培养试验,同时测定针叶失重率和纤维素酶酶活大小。相关性分析表明,内切纤维素酶(endoglucanase,eg)、外切纤维素酶(cellobiohydrolase,cbh)与β-葡萄糖苷酶(β-glucosidase,bgl)三者之间存在一定协同效应(l.pinastri与l.erlangshanense除外),特别是cbh与eg之间。各酶类活性大小是制约针叶纤维素分解的主要因素,协同效应强酶活高凋落针叶分解就越快。5种菌引起针叶质量损失率在8.5%～11.8%之间,各菌株引起针叶质量损失依次为l.pinastri>l.sichuanense>l.kumaunicum>l.conigenum>l.erlangshanense。5种菌均能分泌纤维素酶,对凋落针叶有一定分解能力,且eg、cbh与bgl之间存在不同程度的协同作用,属于兼性寄生物,但其腐生性不强。
| [1] | 宋玉双,何秉章,王福生.我国松落针病研究的新进展[j].森林病虫通讯,1994(2):42-46.songys,hebz,wangfs.newprogressinstudyofneedlecastofconifersinourcountry[j].forestpestanddisease,1994(2):42-46.
|
| [2] | 林英任.松落针病的主要病原―松针散斑壳的研究[j].安徽农学院学报,1987(4):21-28.linyr.studyontheneedlecastfungusofpine,lophodermiumpinastri[j].journalofanhuiagriculturalcollege,1987(4):21-28.
|
| [3] | 刘应高,邱德勋.云南松针上散斑壳的培养类型[j].菌物系统,1997,16(3):224-229.liuyg,qiudx.culturaltypesoflophodermiumofpinusyunnanensisneedles[j].mycosystema,1997,16(3):224-229.
|
| [4] | 刘应高,潘欣,庄启国,等.四川松树6种散斑壳菌记述[j].中国森林病虫,2004,23(4):9-12.liuyg,panx,zhuangqg,etal.sixspeciesoflophodermiumchev.onpinesinsichuanprovince[j].forestpestanddisease,2004,23(4):9-12.
|
| [5] | 刘应高,潘欣,杨静,等.华山松上散斑壳属一新种[j].菌物学报,2009,28(4):473-475.liuyg,panx,yangj,etal.anewspeciesofthegenuslophodermiumonpinusarmandii[j].mycosystema,2009,28(4):473-475.
|
| [6] | 中国林业科学研究院林业研究所.ly/t1271―1999.森林植物与森林枯枝落叶层全氮、磷、钾、纳、钙、镁的测定[s].北京:中国标准出版社,1999.
|
| [7] | 许玉林,郑月霞,叶冰莹,等.一株纤维素降解真菌的筛选及鉴定[j].微生物学通报,2013,40(2):220-227.xuyl,zhengyx,yeby,etal.isolationandidentificationofacellulosedegradingfungi[j].microbiology,2013,40(2):220-227.
|
| [8] | reddyn,yangy.biofibersfromagriculturalbyproductsforindustrialapplications[j].trendsbiotechnol,2005,23(1):22-27.
|
| [9] | 韩业君,陈洪章.植物细胞壁蛋白与木质纤维素酶解[j].化学进展,2007,19(7-8):1153-1158.hanyj,chenhz.plantcellwallproteins&enzymatichydrolysisoflignocellulose[j].progressinchemistry,2007,19(7-8):1153-1158.
|
| [10] | wangls,zhangyz,gaopj,etal.changesinthestructuralpropertiesandrateofhydrolysisofcottonfibersduringextendedenzymatichydrolysis[j].biotechnolbioeng,2006,93(3):443-456.
|
| [11] | 张小梅,李单单,王禄山,等.纤维素酶家族及其催化结构域分子改造的新进展[j].生物工程学报,2013,29(4):422-433.zhangxm,lidd,wangls,etal.molecularengineeringofcellulasecatalyticdomainbasedonglycosidehydrolasefamily[j].chinesejournalofbiotechnology,2013,29(4):422-433.
|
| [12] | 林英任.中国真菌志？第四十卷？斑痣盘菌目[m].北京:科学出版社,2012.
|
| [13] | asaiei,hatak,futaik.effectofsimulatedacidrainontheoccurrenceoflophodermiumonjapaneseblackpineneedles[j].mycologicalresearch,1998,102(11):1316-1318.
|
| [14] | 高国平,王丽丽,祁金玉,等.赤松针叶上4种散斑壳真菌致病性接种试验[j].辽宁林业科技,2009(2):13-15.gaogp,wangll,qijy,etal.inoculationtestonpathogenicityof4lophodermiumspp.onneedleofpinusdensiflora[j].liaoningforestryscienceandtechnology,2009(2):13-15.
|
| [15] | 黄荣雁,王国娟,陈钦华,等.油松常见2种散斑壳属真菌致病性的研究[j].防护林科技,2010(4):39-41.huangry,wanggj,chenqh,etal.pathogenicityof2commonlophodermiumspp.ofpinustabulaeformis[j].protectionforestscienceandtechnology,2010(4):39-41.
|
| [16] | 骆军,刘应高,黄晓丽,等.四川松针上散斑壳菌致病毒素的确定及其致病性差异研究[j].林业科学研究,2010,23(5):685-689.luoj,liuyg,huangxl,etal.pathotoxindeterminationoflophodermiumonpinesinsichuanprovinceandtheirdifferencesinpathogenicity[j].forestresearch,2010,23(5):685-689.
|
| [17] | deckertrj,melvillelh,petersonrl.structuralfeaturesofalophodermiumendophyteduringthecrypticlife-cyclephaseinthefoliageofpinusstrobes[j].mycologicalresearch,2001,105(8):991-997.
|
| [18] | 高国平,王月.油松落针病的病原菌生物学特性及其侵染循环[j].东北林业大学学报,2004,32(6):87-88.gaogp,wangy.biologicalcharacteristicofthepathogenofneedlecastonchinesepineanditsinfectioncycle[j].journalofnortheastforestryuniversity,2004,32(6):87-88.
|
| [19] | 潘欣,刘应高,邹立扣,等.散斑壳属lophodermiumspp.its区的序列分析[j].生物技术,2006,16(4):5-7.panx,liuyg,zoulk,etal.cloningandsequenceingoftheitsregionsoflophodermiumspp.[j].biotechnology,2006,16(4):5-7.
|
| [20] | stenstrome,ihrmarkk.identificationoflophodermiumseditiosumandl.pinastriinswedishforestnurseriesusingspecies-specificpcrprimersfromtheribosomalitsregion[j].forestpathology,2005,35(3):163-172.
|
| [21] | deckertrj,hsiangt,petersonrl.geneticrelationshipsofendophyticlophodermiumnitensisolatesfromneedlesofpinusstrobes[j].mycologicalresearch,2002,106(3):305-313.
|
| [22] | 王士娟,林英任,侯成林,等.基于rdna-its序列的散斑壳属系统发育学初步研究[j].菌物学报,2010,29(2):199-205.wangsj,linyr,houcl,etal.apreliminarystudyonphylogenyoflophodermiumbasedonrdna-itssequences[j].mycosystema,2010,29(2):199-205.
|
| [23] | feios,fonsecan,giganteb,etal.combatingtheparasitelophodermiumseditiosuminnurseriesusingthecompoundmethylcis-7-oxo-deisopropyl-dehydroabietate[j].silvalusitana,1995,3(1):23-36.
|
| [24] | 黄华艳,吴耀军,蓝肖.银杉落针病的初步研究[j].中国森林病虫,2008,27(6):1-3.huanghy,wuyj,lanx.lophodermiumsp.oncathayaargyrophylla[j].forestpestanddisease,2008,27(6):1-3.
|
| [25] | 师光开,罗建堂,侯成林.华山松上的斑痣盘菌[j].菌物学报,2010,29(2):159-163.shigk,luojt,houcl.speciesoftherhytismataceaeonpinusarmandii[j].mycosystema,2010,29(2):159-163.
|
| [26] | koidek,osonot,takedah.colonizationandlignindecompositionofcamelliajaponicaleaflitterbyendophyticfungi[j].mycoscience,2005,46(5):280-286.
|
| [27] | osonot,hirosed.colonizationandlignindecompositionofpineneedlelitterbylophodermiumpinastri[j].forestpathology,2011,41(2):156-162.
|
| [28] | 严海元,辜夕容,申鸿.森林凋落物的微生物分解[j].生态学杂志,2010,29(9):1827-1835.yanhy,guxr,shenh.microbialdecompositionofforestlitter[j].chinesejournalofecology,2010,29(9):1827-1835.
|
| [29] | rowlandap,robertsjd.ligninandcellulosefractionationindecompositionstudiesusingacid-detergentfibermethods[j].communicationsinsoilsciencesandplantanalysis,1994,25(3-4):269-277.
|
| [30] | 中国林业科学研究院林业研究所.ly/t1269―1999.森林植物与森林枯枝落叶层全氮的测定[s].北京:中国标准出版社,1999.
|
| [31] | 中国林业科学研究院林业研究所.ly/t1270―1999.森林植物与森林枯枝落叶层全硅、铝、钙、镁、钾、钠、磷、碳、锰、铜、锌的测定[s].北京:中国标准出版社,1999.
|
| [32] | 全国食品发酵标准化中心.qb2583―2003.纤维素酶制剂[s].北京:中国标准出版社,2003.
|
| [33] | himmelme,dingsy,johnsondk,etal.biomassrecalcitrance:engineeringplantsandenzymesforbiofuelsproduction[j].science,2007,315:804-807.
|
| [34] | vanwyk,jp.biotechnologyandtheutilizationofbiowasteasaresourceforbioproductdevelopment[j].trendsbiotechnol,2001,19(5):172-177.
|
| [35] | 芦光新,陈秀蓉,杨成德,等.真菌分泌纤维素酶与2种草坪草凋落物有机质降解相互关系的研究[j].草地学报,2011,19(6):954-959.lugx,chenxr,yangcd,etal.relationshipbetweencellulosebymicrodochiumbolleyiandtotalorganicmatterdegradationoftwokindsoflawngrasslitter[j].actaagrestiasinica,2011,19(6):954-959.
|
| [36] | osonot.effectsofpriordecompositionofbeechneedlelitterbyphyllospherefungionsubstrateutilizationbyfungaldecomposers[j].mycoscience,2003,44(1):41-45.
|
| [37] | watsond,wilsond,walkerl.synergisminbinarymixturesofthermobifidafuscacellulasescel6b,cel9a,andcel5aonbmccandavicel[j].applbiochembiotechnol,2002,101(2):97-111.
|
| [38] | 陈荣珠,张明亮,蔡少丽,等.浅谈纤维素酶及其应用[j].农产品加工(学刊),2013(4):55-58.chenrz,zhangml,caisl,etal.briefreviewoncellulaseandtheapplicationofcellulose[j].academicperiodicaloffarmproductsprocessing,2013(4):55-58.
|
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