|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
产单宁酶真菌的筛选及产酶条件
DOI: 10.3969/j.jssn.1000-2006.2010.03.002, PP. 6-10
Keywords: 单宁酶,筛选,发酵条件,绿色木霉
Abstract:
从铁冬青等4种植物的新鲜茎、叶组织中分离得到52株真菌，经平板透明圈法初筛得到18株产单宁酶菌株。初筛所得菌株经液体发酵，显示1株木霉属真菌(trichodermaviride)(no.myp07)酶活力最高，达0.248μmol/(min？ml)。采用单因素结合正交实验，得到菌株myp07的最佳发酵条件为：单宁100g/l，蔗糖10g/l，(nh4)2so43g/l，装液量为30ml(250ml锥形瓶)，起始ph为5.0，温度30℃，转速140r/min。在此条件下，菌株my-p-07发酵48h酶活力达到0？392μmol/(min？ml)。
| [1] | 杨顺楷,杨亚力.酶法转化五倍子单宁酸生产没食子酸[j].精细与专用化学品,2005,13(5):12-15.
|
| [2] | aokik,shinker,nishirah.purificationandsomepropertiesoftheyeasttannase[j].agricbiolchem,1976,40:79-89.
|
| [3] | kumarra,gunasekaranp,lakshamananm.biodegradationoftannicacidbycitrobacterfreundiiisolatedfromatanneryeffluent[j].jbasicmicrobiol,1999,39:161-168.
|
| [4] | 宁井铭,方世辉,夏涛,等.酶澄清绿茶饮料研究[j].食品与发酵工业,2005,31(9):122-124.
|
| [5] | 游见明.黑曲霉单宁酶发酵工艺[j].现代食品科技,2005,21(3):96-98.
|
| [6] | 保玉心,邱树毅,李秧针,等.固态发酵黑曲霉产单宁酶发酵条件研究[j].食品与发酵工业,2008,34(6):54-56.
|
| [7] | saxenas,saxenark.statisticaloptimizationoftannaseproductionfrompenicilliumvariableusingfruits(chebulicmyrobalan)ofterminaliachebula[j].biotechnolapplbioc,2004,39:99-106.
|
| [8] | marakiss,diamantoglous.fungiisolationfromleavesofsomemediterraneanevergreensclerophyllousshrubs？enzymatic？activityoftheisolatedfungi[j].cryptogamiemycol,1990,11(4):243-254.
|
| [9] | bajpaib,patils.tanninacylhydrolase(ec3.1.1.20)activityofaspergillus,penicillium,fusariumandtrichoderma[j].worldjmicrobbiot,1996,12(3):217-220.
|
| [10] | 刘小兰,杨顺楷,王忠彦.青霉单宁酶高活性菌株的诱变选育[j].微生物学杂志,2006,26(3):108-110.
|
| [11] | mondalkc,banerjeer,patibr.tannaseproductionbybacilluslicheniformis[j].biotechlett,2000,22:767-769.
|
| [12] | murugans,devipu,maheshp,etal.productionoftannasebycitrobacterfreundiiundersolidstatefermentationanditsapplicationinfruitjuicesdebittering[j].bioscibiotechresasia,2008,5(1):301-306.
|
| [13] | weetallhh.enzymicsynthesisofgallicacidesters[j].applbiochembiotechnol,1985,11(1):25-28.
|
| [14] | 杨亚力,杨维力,刘成君,等.利用黑曲霉单宁酶酶法制取没食子酸的研究[j].天然产物研究与开发,2002,14(2):58-60.
|
| [15] | 宋志萍,蔡俊鹏,曹丽芳.香蕉及茶叶中单宁降解菌株的筛选[j].现代食品科技,2005,21(3):39-41.
|
| [16] | murugank,saravanababus,arunachalamm.screeningoftanninacylhydrolase(e.c.3.1.1.20)producingtanneryeffluentfungalisolatesusingsimpleagarplateandsmfprocess[j].bioresourcetechnol,2007,98:946-949.
|
| [17] | cruz？hernandezm,contrerasjc,liman,etal.productionofaspergillusnigergh1tannaseusingsolid？statefermentation[j].jpureapplmicrobiol,2009,3(1):21-26.
|
| [18] | enemuorsc,odibofjc.cultureconditionsfortheproductionofatannaseofaspergillustamariiimi388810(b)[j].afrjbiotech,2009,8(11):2554-2557.
|
| [19] | banerjeed,patibr.optimizationoftannaseproductionbyaureobasidiumpullulansdbs66[j].jmicrobiolbiotech,2007,17(6):1049-1053.
|
| [20] | 魏景超.真菌鉴定手册[m].上海:上海科学技术出版社,1979.
|
| [21] | costa1am,ribeirowx,katoe,etal.productionoftannasebyaspergillustamariiinsubmergedcultures[j].brazarchbioltechnol,2008,51(2):399-404.
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
