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转Btcry1Ah基因抗虫玉米的分子检测及农艺性状分析

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Keywords: 转基因玉米,种群筛选,分子检测,抗虫,农艺性状

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Abstract:

利用农杆菌介导法将具有自主知识产权的Btcry1Ah基因转入玉米自交系综31中,在获得的1764株转基因植株中筛选获得1株对玉米螟有显著抗性的转基因植株。对该抗性植株进行了室内和田间抗虫性分析及外源基因表达分析,并研究了其杂交后代的农艺性状。结果表明,该转基因植株对亚洲玉米螟田间抗虫性达到高抗水平;外源基因能够正常高效表达,在玉米抽穗期的叶片中Cry1Ah蛋白表达量最高,达到1μg/g鲜重;株高、穗位高、穗长、穗粗等与非转基因对照植株相比差异不大,秃尖长明显短于对照,千粒重及单株产量明显高于对照,这种差异在人工模拟虫害爆发情况下达到极显著水平。

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