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大豆转录因子GmERF5启动子的克隆及活性分析

, PP. 100-104

Keywords: 大豆,GmERF5,GUS活性,启动子,逆境诱导

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Abstract:

乙烯响应因子(Ethylene-responsivefactors,ERFs)广泛参与植物对生物和非生物胁迫的应答。从大豆吉林32的#br#基因组DNA中分离到GmERF5的启动子片段,全长1924bp。该区段含有9种与逆境相关的顺式作用元件,即G-box、GT-1、#br#LTRE-1、W-box、TL1、DPBF、MYB、MYC和BIHD10S。将该启动子构建到植物表达载体pCAMBIA1301上与葡萄糖苷酸酶(GUS)#br#基因融合,注射法转化烟草叶片,并进行干旱、高盐和低温处理,通过GUS组织化学染色和GUS荧光值的测定分析启动子的启动#br#活性。结果表明,在未处理条件下,该启动子能驱动下游GUS基因的表达。干旱和低温处理10h能明显增强GmERF5P的启动活性。

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