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高温短程反硝化菌Brevibacillussp.XF-03特性及其降解动力学

, PP. 177-181

Keywords: Brevibacillussp.XF-03,高温,短程反硝化,亚硝态氮降解动力学,酶活性

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Abstract:

采用梯度驯化方法,使得菌株Brevibacillussp.XF-03在高温(50℃)条件下,能够降解1000mg/L亚硝态氮,并#br#通过单因素试验对其生长碳源和C/N进行优化,结果显示,菌株Brevibacillussp.XF-03短程反硝化最适碳源为琥珀酸钠,C/N为#br#12∶1。在此最佳条件下,42h对初始浓度为100mg/L亚硝态氮去除率为95.1%。对该菌株亚硝态氮降解动力学过程进行模拟,符#br#合基质抑制型的Haldane模型,各参数分别为:最大比降解速率(μmax)=1.28h-1,半饱和常数(KS)=451.42mg/L,底物抑制常#br#数#br#(Ki)=176.77mg/L。初步探讨了亚硝酸盐还原酶(NIR)活性,在该菌株生长指数期的后期,亚硝酸盐还原酶比活力达0.279(U/#br#mgprotein)。

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