全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

一种嵌合型调控元件在肿瘤靶向基因治疗中的应用

DOI: 10.13560/j.cnki.biotech.bull.1985.2015.10.036, PP. 255-262

Keywords: 肿瘤靶向基因治疗,嵌合型表达调控条件,绿色荧光蛋白,单纯疱疹病毒-1胸腺激酶,前列腺癌

Full-Text   Cite this paper   Add to My Lib

Abstract:

肿瘤靶向基因治疗成功的关键是调控治疗基因在肿瘤细胞中特异、高效地表达。首次构建一种嵌合型表达调控元件,旨在转录水平、转录后水平和翻译水平上实现联合调控目的基因在肿瘤细胞中特异性表达。体外实验表明,在前列腺癌细胞系LNCaP中,该调控元件可将报告基因增强型绿色荧光蛋白(EGFP)和荧光素酶(luciferase)的肿瘤表达特异性分别提高420%和480%。体外细胞存活实验表明,运用该元件调控单纯疱疹病毒-1胸腺激酶(HSV-1TK)的表达能特异性杀伤LNCaP细胞,验证了该元件可成功用于治疗基因的肿瘤靶向表达。

References

[1]  Lie-A-Ling M, Bakker CT, Deurholt T, et al. Selection of tumour specific promoters for adenoviral gene therapy of cholangiocarcinoma[J]. J Hepatol, 2006, 44(1):126-133.
[2]  Fang L, Shanqu L, Ping G, et al. Gene therapy with RNAi targeting UHRF1 driven by tumor-specific promoter inhibits tumor growth and enhances the sensitivity of chemotherapeutic drug in breast cancer in vitro and in vivo[J]. Cancer Chemother Pharmacol, 2012, 69(4):1079-1087.
[3]  Wolfe AL, Singh K, Zhong Y, et al. RNA G-quadruplexes cause eIF4A-dependent oncogene translation in cancer[J]. Nature, 2014, 513(7516):65-70.
[4]  DeFatta RJ, Li Y, De Benedetti A. Selective killing of cancer cells based on translational control of a suicide gene[J]. Cancer Gene Ther, 2002, 9(7):573-578.
[5]  Cipollini M, Landi S, Gemignani F. MicroRNA binding site polymorphisms as biomarkers in cancer management and research[J]. Pharmgenomics Pers Med, 2014, 7:173-1791.
[6]  Mazzacurati L, Marzulli M, Reinhart B, et al. Use of miRNA response sequences to block off-target replication and increase the safety of an unattenuated, glioblastoma-targeted oncolytic HSV[J]. Mol Ther, 2015, 23(1):99-107.
[7]  Lai YH, Lin CC, Chen SH, et al. Tumor-specific suicide gene therapy for hepatocellular carcinoma by transcriptionally targeted retroviral replicating vectors[J]. Gene Ther, 2015, 22(2):155-162.
[8]  Kogo R, How C, Chaudary N, et al. The microRNA-218~Survivin axis regulates migration, invasion, and lymph node metastasis in cervical cancer[J]. Oncotarget, 2015, 6(2):1090-1100.
[9]  Hynes NE, Stoelzle T. Key signalling nodes in mammary gland development and cancer:Myc[J]. Breast Cancer Res, 2009, 11(5):210.
[10]  Lang KJ, Kappel A, Goodall GJ. Hypoxia-inducible factor-1alpha mRNA contains an internal ribosome entry site that allows efficient translation during normoxia and hypoxia[J]. Mol Biol Cell, 2002, 13(5):1792-1801.
[11]  Fang Y, Xue JL, Shen Q, et al. MicroRNA-7 inhibits tumor growth and metastasis by targeting the phosphoinositide 3-kinase/Akt pathway in hepatocellular carcinoma[J]. Hepatology, 2012, 55(6):1852-1862.
[12]  Spilka R, Ernst C, Mehta AK, et al. Eukaryotic translation initiation factors in cancer development and progression[J]. Cancer Lett, 2013, 340(1):9-21.
[13]  Moussavi M, Moshgabadi N, Fazli L, et al. Fibroblast growth factor and ornithine decarboxylase 5''UTRs enable preferential expression in human prostate cancer cells and in prostate tumors of PTEN(-/-)transgenic mice[J]. Cancer Gene Ther, 2012, 19(1):19-29.
[14]  Valinezhad Orang A, Safaralizadeh R, Kazemzadeh-Bavili M. Mechanisms of miRNA-mediated gene regulation from common downregulation to mRNA-specific upregulation[J]. Int J Genomics, 2014:970607.
[15]  Li JM, Kao KC, Li LF, et al. MicroRNA-145 regulates oncolytic herpes simplex virus-1 for selective killing of human non-small cell lung cancer cells[J]. Virol J, 2013, 10:241.
[16]  Habibie, Yokoyama S, Abdelhamed S, et al. Survivin suppression through STAT3/β-catenin is essential for resveratrol-induced melanoma apoptosis[J]. Int J Oncol, 2014, 45(2):895-901.
[17]  Paik S, Shak S, Tang G, et al. A multigene assay to predict recurrence of tamoxifen-treated, node-negative breast cancer[J]. N Engl J Med, 2004, 351(27):2817-2826.
[18]  Meijer TW, Kaanders JH, Span PN, et al. Targeting hypoxia, HIF-1, and tumor glucose metabolism to improve radiotherapy efficacy[J]. Clin Cancer Res, 2012, 18(20):5585-5594.
[19]  Shi Y, Frost P, Hoang B, et al. MNK kinases facilitate c-myc IRES activity in rapamycin -treated multiple myeloma cells[J]. Oncogene, 2013, 32(2):190-197.
[20]  Sujobert P, Bardet V, Cornillet-Lefebvre P, et al. Essential role for the p110delta isoform in phosphoinositide 3-kinase activation and cell proliferation in acute myeloid leukemia[J]. Blood, 2005, 106(3):1063-1066.
[21]  Foka P, Pourchet A, Hernandez AR, et al. Novel tumour-specific promoters for?transcriptional?targeting?of hepatocellular carcinoma by herpes simplex virus vectors[J]. J Gene Med, 2010, 12(12):956-967.
[22]  Lukowski SW, Rothnagel JA, Trezise AE. CFTR mRNA expression is regulated by an upstream open reading frame and RNA secondary structure in its 5'' untranslated region[J]. Hum Mol Genet, 2015, 24(4):899-912.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133