全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

绿针假单胞菌HT66中luxR_19对吩嗪-1-甲酰胺合成的影响

DOI: 10.13560/j.cnki.biotech.bull.1985.2015.08.024, PP. 166-173

Keywords: 绿针假单胞菌,HT66,吩嗪-1-甲酰胺,LuxR家族转录调控因子

Full-Text   Cite this paper   Add to My Lib

Abstract:

旨在研究调控基因luxR_19对绿针假单胞菌HT66中的抑菌物质吩嗪-1-甲酰胺(phenazine-1-carboxamide,PCN)合成及菌体生长的影响,通过分子操作构建了luxR_19基因敲除株、回补菌株、过表达菌株以及luxR_19(G85A)点突变菌株,检测了目标菌株生长曲线、PCN发酵结果、细菌泳动性与从动性等。结果显示,将luxR_19敲除后PCN产量明显降低,对luxR_19基因进行过表达,发现能够将PCN产量提高2倍;luxR_19对于吩嗪合成的多个调控基因以及合成基因表达均产生影响;luxR_19的敲除对HT66的生长及泳动性不产生影响,而对细菌的从动性有一定的促进作用;luxR_19(G85A)点突变菌株吩嗪产量较野生型相差很小。结果表明,luxR_19对PCN的合成为正调控基因,一定程度上影响HT66的从动性,第254位碱基突变对PCN合成产量不产生影响。

References

[1]  张锋华, 许煜泉, 张雪洪.农用杀菌剂吩嗪-1-羧酸的生物合成与基因调控[J].农药研究与应用, 2006(5):4-7.
[2]  Han SH, Lee SJ, Moon JH, et al. GacS-dependent production of 2R, 3R-butanediol by Pseudomonas chlororaphis O6 is a major determinant for eliciting systemic resistance against Erwinia carotovora but not against Pseudomonas syringae pv. tabaci in tobacco[J]. Mol Plant-Microbe Interact, 2006, 19(8):924-930.
[3]  Chin-A-Woeng TF, Bloemberg GV, van der Bij AJ, et al. Biocontrol by phenazine-1-carboxamide-producing Pseudomonas chlororaphis PCL1391 of tomato root rot caused by Fusarium oxysporum f. sp. radicis-lycopersici[J]. Mol Plant-Microbe Interact, 1998, 11(11):1069-1077.
[4]  Shen X, Hu H, Peng H, et al. Comparative genomic analysis of four representative plant growth-promoting rhizobacteria in Pseudomonas[J]. BMC Genomics, 2013, 14(1):271.
[5]  Loper JE, Hassan KA, Mavrodi DV, et al. Comparative genomics of plant-associated Pseudomonas spp.:insights into diversity and inheritance of traits involved in multitrophic interactions[J]. PLoS Genet, 2012, 8(7):e1002784.
[6]  Park J, Oh S, Anderson A, et al. Production of the antifungal compounds phenazine and pyrrolnitrin from Pseudomonas chlororaphis O6 is differentially regulated by glucose[J]. Lett Appl Microbiol, 2011, 52(5):532-537.
[7]  Maddula V, Pierson E, Pierson L. Altering the ratio of phenazines in Pseudomonas chlororaphis(aureofaciens)strain 30-84:effects on biofilm formation and pathogen inhibition[J]. J Bacteriol, 2008, 190(8):2759-2766.
[8]  张平原, 彭华松, 张雪洪.高产吩嗪-1-甲酰胺的绿针假单胞菌的诱变与基因工程育种[J].上海交通大学学报:农业科学版, 2015, 33(2):90-94.
[9]  Whistler CA, Pierson III LS. Repression of phenazine antibiotic production in Pseudomonas aureofaciens strain 30-84 by RpeA[J]. J Bacteriol, 2003, 185(13):3718-3725.
[10]  张云, 胡洪波, 彭华松, 等.产PCA基因工程菌M18G反复补料分批培养研究[J].工业微生物, 2007:16-20.
[11]  Liu H, He Y, Jiang H, et al. Characterization of a phenazine-producing strain Pseudomonas chlororaphis GP72 with broad-spectrum antifungal activity from green pepper rhizosphere[J]. Curr Microbiol, 2007, 54(4):302-306.
[12]  Huang L, Chen MM, Wang W, et al. Enhanced production of 2-hydroxyphenazine in Pseudomonas chlororaphis GP72[J]. Appl Microbiol Biotechnol, 2011, 89(1):169-177.
[13]  He W, Lei J, Jiu Y, et al. The LuxR family members gdmri and gdmrii are positive regulators of geldanamycin biosynthesis in Streptomyces hygroscopicus 17997[J]. Arch Microbiol, 2008, 189(5):501-510.
[14]  Fernández-piñar R, Cámara M, Soriano MI, et al. Ppor, an orphan luxr-family protein of Pseudomonas putida KT2440, modulates competitive fitness and surface motility independently of n-acylhomoserine lactones[J]. Environmental Microbiology Reports, 2011, 3(1):79-85.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133