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4种油脂对瘤胃微生物体外产气及辅酶F420的影响

DOI: 10.3969/j.issn.1006-267x.2011.10.024

Keywords: 油脂,瘤胃微生物,甲烷,辅酶F420,产气量

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Abstract:

本文主要研究不同饱和程度油脂对瘤胃微生物体外产气及辅酶F420动态变化的影响。试验选用3头装有瘤胃瘘管的徐淮白山羊提供瘤胃液,分对照组、花生油组(A)、菜籽油组(B)、玉米油组(C)和豆油组(D)5个组进行体外培养,每组3个重复,每个重复1个培养瓶。结果表明:1)36h总产气量在20.61~39.67mL间变化,除花生油组外,其他油脂组均显著低于对照组(P<0.05);随时间的延长,各组产气量呈现波动变化,甲烷产量和甲烷/总产气量比率在组间差异显著(P<0.05),且甲烷/总产气量比率有从B到D逐渐下降的趋势。2)对照组辅酶F420荧光值显著高于油脂组(P<0.05),除菜籽油组外,其他油脂组辅酶F420荧光值有随油脂不饱和程度增加而降低的趋势。3)相关分析结果表明,辅酶F420荧光值与甲烷产量(0.916)、与总产气量(0.905)相关皆极显著(2-尾<0.01)。4)豆油组总脱氢酶显著高于对照组,并依次显著高于玉米油、花生油、菜籽油组(P<0.05)。因此,随本试验所选油脂不饱和程度的加大,抑制甲烷产量和总产气量的效应也随之加大,并以豆油的效果较佳。

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