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不同方法测定瘤胃非降解蛋白质小肠消化率及相关性分析

DOI: 10.3969/j.issn.1006-267x.2012.07.011

Keywords: 蛋白质饲料,能量饲料,相关性分析,小肠消化率

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Abstract:

本试验分别采用移动尼龙袋法、改进三步体外法、原始三步体外法和酸性洗涤不溶氮(ADIN)估测法测定13种精料的瘤胃非降解蛋白质(RUP)小肠消化率,并对移动尼龙袋法与其他方法的测定结果进行相关性分析,同时对常规营养成分和RUP小肠消化率作回归分析,旨在寻找替代移动尼龙袋法测定RUP小肠消化率更简单快速的方法。试验采用单因素试验设计,选取3头装有永久瘤胃瘘管和十二指肠T型瘘管的奶牛。结果表明:移动尼龙袋法测得的豆粕、棉籽粕、菜籽粕、葵花粕、芝麻粕、玉米胚芽粕、玉米、米糠、米糠粕、米糠饼、大麦、麦麸和玉米麸质饲料的RUP小肠消化率分别为98.13%、87.37%、88.47%、82.60%、85.73%、75.40%、93.23%、69.27%、77.83%、92.10%、91.27%、72.37%和79.03%。改进三步体外法测定结果与移动尼龙袋法测定结果的相关系数为0.8383;原始三步体外法测定结果与移动尼龙袋法测定结果的相关系数为0.7899;ADIN估测法预测结果与移动尼龙袋法测定结果无显著相关(P>0.05);饲料RUP小肠消化率与饲料常规营养成分含量显著相关(P<0.05)。改进三步体外法比原始三步体外法替代移动尼龙袋法测定饲料RUP小肠消化率效果更好。应用体外法替代移动尼龙袋法测定蛋白质饲料RUP小肠消化率比测定能量饲料RUP小肠消化率效果更好。用饲料的常规营养成分含量可较好地预测RUP小肠消化率。

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