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富马酸亚铁对虹鳟幼鱼生长性能、血液生化指标及微量元素含量的影响

DOI: 10.3969/j.issn.1006-267x.2012.04.018

Keywords: 虹鳟,富马酸亚铁,血液生化指标,微量元素

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Abstract:

本试验旨在研究富马酸亚铁对虹鳟幼鱼生长性能、血液生化指标及微量元素含量的影响。选择初始体重为(89.2±0.2)g的虹鳟幼鱼360尾,随机分成6组,每组3个重复,每个重复20尾鱼,分别饲喂添加富马酸亚铁0、20、40、80、160、480mg/kg(以铁计)的试验饲料,各组分别为D0、D20、D40、D80、D160和D480组,D0组为对照组。各组铁实测含量分别为62.6、79.5、99.6、139.3、215.2、538.4mg/kg,试验期60d。结果表明:1)饲料中不同铁水平对虹鳟幼鱼生长性能无显著影响(P>0.05)。2)血红蛋白含量和红细胞数量呈现先上升后平稳的趋势;各组血细胞比容无显著差异(P>0.05)。3)各组全鱼、脊椎骨和肌肉铁含量随着饲料中铁水平的升高而升高,肝脏铁含量呈现先上升后平稳的趋势;各组全鱼锌含量无显著差异(P>0.05),D0组脊椎骨和肌肉锌含量显著高于其他各组(P<0.05),D0和D20组肝脏锌含量显著高于其他各组(P<0.05);全鱼铜含量随饲料铁含量的升高而升高,各组脊椎骨铜含量无显著差异(P>0.05),D0组肌肉铜含量显著低于其他各组(P<0.05)。4)D0组血清溶菌酶活性显著低于D20、D40、D80和D160组(P<0.05),但与D480组无显著差异(P>0.05);过氧化氢酶活性呈现先上升后下降趋势,D40组最高。由此可见,本试验条件下,以富马酸亚铁作为铁源,通过折线模型分析发现,以血红蛋白含量和肝脏铁含量为评价指标的虹鳟幼鱼对饲料中铁需要量分别为99.8和100.4mg/kg。

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