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饲粮中添加苏氨酸和色氨酸对接种猪繁殖与呼吸综合征弱毒苗生长猪免疫反应的影响

DOI: 10.3969/j.issn.1006-267x.2013.06.010

Keywords: 苏氨酸,色氨酸,PRRS弱毒苗,免疫反应,生长猪

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Abstract:

本试验旨在研究饲粮中添加苏氨酸和色氨酸对接种猪繁殖与呼吸综合征(PRRS)弱毒苗生长猪免疫反应的影响。试验选取16头平均体重为(29.11±4.41)kg健康去势生长猪,按体重相近原则随机分为4个处理,每个处理4个重复,每个重复1头猪。采用2×2两因子试验设计,设2个苏氨酸和色氨酸添加水平,按比例分别为赖氨酸:苏氨酸:色氨酸=100:64:18(NRC对照水平)和赖氨酸:苏氨酸:色氨酸=100:80:26(试验水平);2个氨基酸添加水平下按免疫处理情况分为注射磷酸盐缓冲液(PBS)和接种PRRS弱毒苗(试验开始当天和第35天肌肉注射2mLPBS或PRRS弱毒苗)。试验第14、28、35和49天收集血样,检测血浆中干扰素-γ(IFN-γ)、白细胞介素-10(IL-10)、白细胞介素-1β(IL-1β)含量和血清中免疫球蛋白G(IgG)含量;试验第49天屠宰取样,利用实时荧光定量PCR(RT-qPCR)方法检测肺组织中Toll样受体1~10(TLR1~TLR10)mRNA相对表达量。结果表明:与饲粮添加NRC对照水平苏氨酸和色氨酸相比,注射PBS条件下,饲粮添加试验水平苏氨酸和色氨酸显著提高了生长猪试验第14和28天血清IgG含量(P<0.05);接种PRRS弱毒苗条件下,饲粮添加试验水平苏氨酸和色氨酸显著提高了生长猪试验第35天血清IgG含量(P<0.05)。试验第14、28和35天,接种PRRS弱毒苗生长猪血浆IFN-γ、IL-10、IL-1β含量均显著高于注射PBS生长猪(P<0.05);试验第14、28、35和49天,接种PRRS弱毒苗条件下,与饲粮添加NRC对照水平苏氨酸和色氨酸相比,饲粮添加试验水平苏氨酸和色氨酸提高了生长猪血浆IL-1β(试验第14天除外)、IFN-γ(试验第49天除外)含量,降低了血浆IL-10含量,但差异均不显著(P>0.05)。接种PRRS弱毒苗生长猪肺组织中TLR1~TLR7、TLR9、TLR10mRNA相对表达量显著高于注射PBS生长猪(P<0.05);接种PRRS弱毒苗条件下,与饲粮添加NRC对照水平苏氨酸和色氨酸相比,饲粮添加试验水平苏氨酸和色氨酸显著提高了生长猪肺组织中TLR1~TLR3、TLR7、TLR8、TLR10mRNA相对表达量(P<0.05),显著降低了TLR4、TLR6、TLR9mRNA相对表达量(P<0.05)。由结果分析可知,饲粮中添加苏氨酸和色氨酸可上调TLR3、TLR7、TLR8mRNA相对表达量,表明PRRS弱毒苗可能激活了TLR3、TLR7、TLR8信号通路,进而提高血清免疫球蛋白含量,促进炎症细胞因子产生,最终强化获得性免疫。

References

[1]  ASTE-AMEZAGA M,MA X,SARTORI A.Molecular mechanisms of the induction of IL-12 and its inhibition by IL-10[J].The Journal of Immunology,1998,160:5936-5944.
[2]  LIU C H,CHAUNG H C,CHANG H L,et al.Expression of Toll-like receptor mRNA and cytokines in pigs infected with porcine reproductive and respiratory syndrome virus[J].Veterinary Microbiology,2009,136:266-276.
[3]  MIGUEL J C,CHEN J,VAN ALSTINE W G,et al.Expression of inflammatory cytokines and Toll-like receptors in the brain and respiratory tract of pigs infected with porcine reproductive and respiratory syndrome virus[J].Veterinary Immunology and Immunopathology,2010,135(3):314-319.
[4]  ARANCIBIA S A,BELTRN C J,AGUIRRE I M,et al.Toll-like receptors are key participants in innate immune responses[J].Biological Research,2007,40(2):97-112.
[5]  DIEBOLD S S,KAISHO T,HEMMI H,et al.Innate antiviral responses by means of TLR7 mediated recognition of single-stranded RNA[J].Science,2004,303(5):1529-1531.
[6]  ALEXOPOULOU L,HOLT C,MEDZHITOV R,et al.Recognition of double-stranded RNA and activation of NF-κB by Toll-like receptor 3[J].Nature,2001,413:732-738.
[7]  MEDZHITOV R.Toll-like receptors and innate immunity[J].Nature Reviews Immunology,2001,1(2):135-145.
[8]  POLTORAK A,SMIRNOVA I,HE X,et al.Genetic and physical mapping of the LPS locus:identification of toll-4 receptor as a candidate gene in the critical region[J].Blood Cells,Molecules,and Diseases,1998,24:340-355.
[9]  DIAZ I,DARWICH L,PAPPATERRA G,et al.Different european-type vaccines against porcine reproductive and respiratory syndrome virus have different immunological properties and confer different protection to pigs[J].Virology,2006,351:249-259.
[10]  THANAWONGNUWECH A,UNGSIPIPAT S,DISATIAN R,et al.Immunohistochemical staining of IFN-γ positive cells in porcine reproductive and respiratory syndrome virus-infected lungs[J].Veterinary Immunology and Immunopathology,2003,91:73-77.
[11]  VAN REETH K,LABARQUE G,NAUWYNCK H.Differential production of proinflammatory cytokines in the pig lung during different respiratory virus infections:correlations with pathogenicity[J].Research in Veterinary Science,1999,67:47-52.
[12]  SURADHAT S,THANAWONGNUWECH R.Upregulation of interleukin-10 gene expression in the leukocytes of pigs infected with porcine reproductive and respiratory syndrome virus[J].Journal of General Virology,2003,84:2755-2760.
[13]  THANAWONGNUWECH R,THACKER B,HALBUR P.Increased production of proinflammatory cytokines following infection with porcine reproductive and respiratory syndrome virus and mycoplasma hyopneumoniae[J].Clinical and Diagnostic Laboratory,2004,11:901-908.
[14]  CHAUNG H C,PENG Y T,CHANG H L,et al.Phenotypic and functional modulation of bone marrow-derived dendritic cells by porcine reproductive and respiratory syndrome virus[J].Veterinary Microbiology,2008,129:281-293.
[15]  MEIER W A,GALEOTA J,OSORIO F A,et al.Gradual development of the interferon-gamma response of swine to porcine reproductive and respiratory syndrome virus infection or vaccination[J].Virology,2003,309:18-31.
[16]  NAGASE H,OKUGAWA S,OTA Y,et al.Expression and function of toll-like receptors in eosinophils:activation by Toll-like receptor 7 ligand[J].The Journal of Immunology,2003,171(8):3977-3982.
[17]  SCHNARE M,BARTON G,HOLT A C,et al.Toll-like receptors control activation of adaptive immune responses[J].Nature Immunology,2001,2:947-950.
[18]  CANO J P,DEE S A,MURTAUGH M P,et al.Effect of vaccination with a modified-live porcine reproductive and respiratory syndrome virus vaccine on dynamics of homologous viral infection in pigs[J].American Journal of Veterinary Research,2007,68:565-571.
[19]  胡家澄,邹晓庭,董金格.苏氨酸对畜禽免疫性能的影响研究进展[J].中国饲料,2008(21):4-6.
[20]  郑春田,李德发,谯仕彦,等.生长猪苏氨酸需要量研究[J].畜牧与兽医,2000,32(1):9-11.
[21]  WANG X,QIAO S Y,LIU M,et al.Effects of graded levels of true ileal digestible threonine on performance,serum parameters and immune function of 10-25 kg pigs[J].Animal Feed Science and Technology,2006,129:264-278.
[22]  LI D,XIAO C,QIAO S,et al.Effects of dietary threonine on performance,plasma parameters and immune function of growing pigs[J].Animal Feed Science and Technology,1999,78:179-188.
[23]  FALARINO F,GROHMANN U,YOU S,et al.The combined effects of tryptophan starvation and tryptophan catabolites down regulate T cell receptor chain and induce a regulatory phenotype in naive T cells[J].The Journal of Immunology,2006,76:6752-6761.
[24]  MURTAUGH M P,XIAO Z,ZUCKERMANN F.Immunological responses of swine to porcine reproductive and respiratory syndromevirus infection[J].Viral Immunology,2002,15:533-547.
[25]  OSORIO F A,GALEOTA J A,NELSON E,et al.Passive transfer of virus-specific antibodies confers protection against reproductive failure induced by a virulent strain of porcine reproductive and respiratory syndrome virus and establishes sterilizing immunity[J].Virology,2002,302:9-20.
[26]  MILLER L C,LAGER K M,KEHRLI M E.Role of toll-like receptors in activation of porcine alveolar macrophages by porcine reproductive and respiratory syndrome virus[J].Clinical and Vaccine Immunology,2009,16:360-365.
[27]  LUO R,XIAO S,JIANG Y,et al.Porcine reproductive and respiratory syndrome virus (PRRSV) suppresses interferon-beta production by interfering with the RIG-Ⅰ signaling pathway[J].Molecular Immunology,2008,45:2839-2846.
[28]  SANG Y,ROSS C R,ROWLAND R R,et al.Toll-like receptor 3 activation decreases porcine arterivirus infection[J].Molecular Immunology,2008,21:303-314.

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