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生长激素和胰岛素样生长因子Ⅰ对奶牛乳蛋白合成关键激酶及调节因子mRNA表达量的影响

DOI: 10.3969/j.issn.1006-267x.2013.01.025

Keywords: 生长激素,胰岛素样生长因子Ⅰ,乳腺上皮细胞,乳蛋白,mRNA表达量

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Abstract:

本试验旨在探讨生长激素(GH)和胰岛素样生长因子Ⅰ(IGF-Ⅰ)对体外培养的奶牛乳腺上皮细胞内调控乳蛋白合成的关键激酶及调节因子mRNA表达量的影响。试验对纯化后的荷斯坦奶牛乳腺上皮细胞进行4种处理,对照组采用无血清生长培养基,试验组在对照组的基础上分别添加GH(100ng/mL)、IGF-Ⅰ(100ng/mL)和GH(100ng/mL)+IGF-Ⅰ(100ng/mL)。培养24h后,采用实时定量PCR(RT-qPCR)法测定κ-酪蛋白基因以及调控乳蛋白合成的关键激酶及调节因子的mRNA表达量,并测定生长激素受体(GHR)和胰岛素样生长因子Ⅰ受体(IGF-ⅠR)mRNA表达量。结果表明:体外培养的奶牛乳腺上皮细胞可以表达GHR和IGF-ⅠRmRNA,各试验组均能显著提高κ-酪蛋白(CSN3)mRNA表达量(P<0.05),但未发现GH和IGF-Ⅰ复合存在累积效应;与对照组相比,GH组有提高E74-样转录因子5(ELF5)mRNA表达量的趋势(P<0.10),而IGF-Ⅰ组显著提高了哺乳动物雷帕霉素靶蛋白(mTOR)和核糖体蛋白S6激酶1(rpS6K1)mRNA表达量(P<0.05),GH+IGF-Ⅰ组未呈现加强作用。结果提示,GH和IGF-Ⅰ可能单独通过影响调控乳蛋白合成的关键激酶及调节因子mRNA表达来调节κ-酪蛋白的合成。

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