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用于紫菜无性系种质鉴定的计算机化DNA指纹的建立

, PP. 79-84

Keywords: 计算机化的DNA指纹,种质鉴定,RAPD,紫菜

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Abstract:

研究用RAPD技术对条斑紫菜(Porphyrayezoensis)、坛紫菜(P.Haitanensis)、半叶紫菜(P.Katadaivar.Hemiphylla)和少精紫菜(P.Oligospermatangia)的15个无性系丝状体进行了遗传多样分析,用120个Operon引物进行了筛选,从中选用来自OPJ-18和OPN-02扩增出的8条多态性条带构建了这15个紫菜无性系的DNA指纹图谱,在该图谱中每个紫菜无性系均有各自特异的DNA指纹.用1和0分别表示DNA带的出现和缺失,将各个无性系的DNA指纹用计算机语言表示,建立了15个紫菜无性系的计算机化DNA指纹;在此基础上设计了紫菜无性系种质鉴定的计算机软件PhGI.

References

[1]  NOLI E, CONTI S, MACCAFERRI M, et al. Molecular characterization of tomatocultivars [J]. Seed Sci & Technol,1999, 27: 1~10.
[2]  王斌,张超良,翁曼丽,等.玉米自交系8112特异SCAR标记的获得[J].高技术通讯,1999,9(3):45~47
[3]  DUTCHER J A, KAPRAUN D F. Random amplified polymorphic DNA (RAPD) identification ofgenetic variation in three species of Porphyra (Bangiales, Rhodophyta) [J]. Journal ofApplied Phycology, 1994, 6: 267~273.
[4]  PATWARY M U, MACKAY R M, VAN DER MEER J P. Revealing genetic markers in Gelidiumvagum (Rhodophyta)through the random amplified polymorphic DNA (RAPD) technique [J].Journal of Phycology, 1993,29: 216~222.
[5]  HO C L, PHANG S M, PANG T. Molecular characterisation of Sargassum polycystum andS. siliquosum (Phaeophyta)by polymerase chain reaction (PCR) using random amplifiedplymorphic DNA (RAPD) primers [J]. J Appl Phycol, 1995,7(1): 37~41.
[6]  CHEN Yi-hua, JIA Jian-hang, LI Chuan-you, et al. Rice seed identification bycomputerized AFLP-DNA fingerprinting[J]. Chinese Rice Research Newsletter, 2000, 8(1): 4~5.
[7]  SELBACH A, CAAVALLI-MOLINA S. RAPDcharacterization of Brazilian barley (Hordeum vulgare ssp. vulgare) varieties [J].Euphytica, 2000, 111: 127~135.
[8]  ARIFIN NS, OZAKI Y, OKUBO H. Genetic diversity in indonesian shallot (Allium cepavar. ascalonicum) and Allium ×wakegi revealed by RAPD markers and origin of A. ×wakegiidentified by RFLP analysis of amplified chloroplast genes[J]. Euphytica, 2000, 111: 23~31.
[9]  JIA Jian-hang, WANG Ping, JIN De-min, et al. The application of RAPD markers indiversity detection and variety identification of porphyra [J]. Acta Botanica Sinica,2000, 42(4): 403~407.
[10]  PATWARY M U, VAN DER MEER J P. Aplication of RAPD markers in an examination ofheterosis in Gelidium vagum (Rhodophyta) [J]. Journal of Phycology, 1994, 30: 91~97.

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