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外源激素对养殖牙鲆血浆睾酮和雌二醇含量的影响研究

, PP. 115-120

Keywords: 牙鲆,性激素,生殖

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Abstract:

雄性牙鲆单独注射促黄体激素释放激素类似物(LHRH-A)(每千克质量的注射量,下同)25μg/kg或人绒毛膜促性腺激素(hCG)1000IU/kg,血浆睾酮(T)含量在注射后6h大幅度降低,12h时达到最低值,以后逐渐回升;LHRH-A与hCG联合注射[LHRH-A(12.5μg/kg)+hCG(500IU/kg)],注射后6h血浆T含量剧烈下降,24h时达到最低点,以后缓慢回升;对照组雄鱼血浆T含量随着采样进行而呈现降低趋势.雌鱼单独LHRH-A后,血浆雌二醇(E2)水平逐渐下降,24h时达到最低值,30h后略有回升;单独注射hCG后,血浆E2含量缓慢下降,30h达到最低值;LHRH-A与hCG联合注射,注射后6h血浆E2含量逐渐下降,24h时达到最低点,30h时缓慢回升.对照组雌鱼血浆E2水平也随着采样的进行而逐渐降低.结果表明:单独或联合注射LHRH-A和hCG降低了雌、雄牙鲆血浆E2和T含量,与对照组结果相同.

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