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检测犬源狂犬病毒中和抗体ELISA方法的建立

DOI: 10.7671/j.issn.1001-411X.2012.04.026, PP. 561-565

Keywords: 狂犬病毒,糖蛋白,犬源,中和抗体,间接ELISA

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Abstract:

为了有效监测犬免疫狂犬病疫苗后的保护效力,以狂犬病毒(Rabiesvirus,RV)糖蛋白的主要优势抗原表位区G3蛋白(RVG3)作为包被抗原,建立了一种检测狂犬病毒中和抗体效价的间接ELISA方法.通过优化反应条件,确定抗原最佳包被量为8mg/L,血清的最佳稀释度为1∶100,酶标二抗的稀释度为1∶3000.特异性试验表明,该抗原不与犬瘟热病毒、犬腺病毒、犬细小病毒阳性血清发生交叉反应;批内和批间重复性试验的平均变异系数都小于10%;敏感性达1∶1280.此方法检测134份血清样品的结果与美国SYNBIOTICS试剂盒相比,总符合率达95.6%.

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