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小麦株高近等基因系的RAPD标记研究

DOI: 10.3321/j.issn:1000-7091.2000.01.002, PP. 7-11

Keywords: 小麦,基因标记,rht基因,RAPD

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Abstract:

采用RAPD技术,以4个小麦株高基因近等基因系(分别含有rht、Rht1、Rht2、Rht3)为材料,对296个单一随机引物(10个核苷酸)进行了筛选。发现25个引物的扩增产物在近等基因系间表现出特异性。在6次重复试验中,有18个引物的特异扩增片段不能重复,6个引物可以重复2~3次,唯有OPAM01在全部试验中均能稳定重复,其特异扩增片段OPAM011860可以作为rht基因的RAPD标记。

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