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猪繁殖与呼吸综合征的套式PCR方法建立及应用

DOI: 10.7668/hbnxb.2009.01.026, PP. 115-118

Keywords: 猪繁殖与呼吸综合征病毒,套式PCR方法,建立和应用

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Abstract:

通过设计2对引物,建立了PRRSV的套式PCR方法,PRRSV细胞毒稀释10000倍,依然能扩增出相应的片断,多次重复能得到一致的结果,非PRRSV病毒(猪瘟病毒、猪乙型脑炎病毒)均未扩增出相应片断。应用该方法对各地样品进行检测,表明建立的套式PCR方法适合进行流行病学调查。

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