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转基因地被菊晚粉抗旱生理特性研究

DOI: 10.7668/hbnxb.2011.05.036, PP. 181-185

Keywords: 转基因,地被菊,OtMYB,干旱胁迫,生理特性

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Abstract:

对转太行菊OtMYB基因晚粉进行干旱胁迫和复水处理,以野生型晚粉和野生太行菊为对照,测定不同水分胁迫下叶片的叶绿素、可溶性蛋白、丙二醛、游离脯氨酸、可溶性糖的含量和膜透性,比较外源OtMYB基因表达对转基因晚粉抗旱性的影响。结果表明:随胁迫时间的延长,三者叶片中叶绿素含量均呈下降趋势,丙二醛含量、游离脯氨酸含量和相对电导率明显上升,但转基因晚粉各指标变化规律与太行菊相似,比野生型晚粉表现出更强的稳定性和恢复能力,表明OtMYB基因在转基因植株中成功表达,并提高了植株的抗旱性。转基因植株的可溶性蛋白和可溶性糖含量,表现出与OtMYB基因来源种太行菊相似的变化趋势,明显区别于野生型晚粉,推测OtMYB基因在干旱胁迫下能诱导转基因植株糖和蛋白的特异表达。

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