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CdCl2对牛卵母细胞体外成熟及凋亡的影响

DOI: 10.7668/hbnxb.2011.05.030, PP. 146-152

Keywords: CdCl2,牛卵母细胞,体外成熟,凋亡

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Abstract:

利用不同浓度(0,0.8,1.6,2.4μg/mL)的氯化镉(CdCl2)对处理牛卵母细胞前后对其成熟率、凋亡率、Ca2+分布、线粒体膜电位、Bcl-2与Bax的基因表达的影响,初步为重金属对雌性哺乳动物生殖细胞发育及细胞凋亡机制的进一步研究打下基础。利用0,0.8,1.6,2.4μg/mL的氯化镉(CdCl2)处理牛卵母细胞22h,统计其凋亡率及死亡率;利用AnnexinV-FITC检测牛卵母细胞的早期凋亡;分别用Fluo-3/AM和Rh123检测卵母细胞的Ca2+分布及其线粒体膜电位的变化;利用RT-PCR检测促凋亡基因Bcl-2和抑凋亡基因Bax的相对表达。经过上述处理后,0.8μg/mL处理组卵母细胞的成熟率和凋亡率与对照组相比差异不显著(P>0.05),而1.6μg/mL和2.4μg/mL处理组与对照组相比差异显著(P<0.05);随着处理浓度的增加,胞浆Ca2+的荧光强度逐渐增强,线粒体膜电位逐渐下降,bcl-2与Bax的相对比值下降。CdCl2诱导卵母细胞的凋亡呈剂量效应,可以抑制卵母细胞的成熟,促进细胞的凋亡。

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