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天津地区保护地蔬菜根结线虫种类的分子鉴定

DOI: 10.3969/j.issn.1000-7091.2012.05.034, PP. 179-183

Keywords: 蔬菜,根结线虫,线粒体DNA,限制性片段长度多态性,内转录间隔区

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Abstract:

旨为明确天津地区蔬菜根结线虫的主要种类,为该区域线虫病害的防治提供理论依据。从天津市7个区、县温室中采集蔬菜根结线虫样本14份,应用线粒体DNA-限制性片段长度多态性(mtDNA-RFLP)技术,结合核糖体DNA内转录间隔区(rDNA-ITS区)的序列分析对其进行种类鉴定。结果表明,在天津地区采集的蔬菜根结线虫均为南方根结线虫。

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