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牦牛VEGF—A基因的克隆及生物信息学分析

DOI: 10.3969/j.issn.1000-7091.2012.04.009, PP. 48-53

Keywords: 牦牛,VEGF&mdash,A基因,克隆,生物信息学分析

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Abstract:

利用分子克隆技术获得了牦牛VEGF-A基因编码区序列,并采用生物信息学方法对该基因及其编码蛋白的基本理化性质、疏水性、信号肽、二级结构等方面进行了预测和分析。结果表明,牦牛VEGF—A基因包含一个573bp的开放阅读框,编码190个氨基酸;其编码蛋白属于亲水性蛋白,具有明显的信号肽。二级结构主要以无规则卷曲和α螺旋为主。VEGF—A基因编码产物氨基酸邻接系统树表明,牦牛VEGF—A与黄牛、绵羊和成都麻羊等物种的VEGF—A氨基酸遗传距离较近,具有高度同源性。

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