全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

山药种质包埋玻璃化超低温保存再生植株的稳定性分析

DOI: 10.3969/j.issn.1000-7091.2012.01.044, PP. 234-238

Keywords: 山药,超低温保存,包埋玻璃化法,稳定性

Full-Text   Cite this paper   Add to My Lib

Abstract:

为了验证前期建立的包埋玻璃化超低温保存技术是山药(Dioscoreaopposita)种质资源离体保存的一种有效的方法,对包埋玻璃化超低温保存后的山药再生植株的形态、生理和同工酶酶谱进行了研究。结果表明:与常温保存的植株相比,供试的铁棍山药再生植株的平均株高、叶片数、芽数和茎节长,叶片中叶绿素、可溶性蛋白和可溶性糖含量以及超氧化物歧化酶(SOD)活力在绝对值上虽有差异,但均未达到显著水平,且能移栽成活,保持了形态和生理的稳定性;供试的铁棍、太谷、怀庆和嘉祥细长毛4种山药再生植株的SOD和蛋白水解酶的同工酶条带数目及强度均未发生变化,保持了酶蛋白的稳定性。说明前期建立的包埋玻璃化法超低温保存技术是山药种质离体保存的一种有效方法。

References

[1]  Hirai D,Shirai K,Shirai S,et al.Cryopreservation of in vitro-grown meristems of strawberry(Fragaria × ananassa Duch.) by encapsulation-vitrification[J] Euphytica,1998,101:109-115.
[2]  Paul H,Daigny G,Sangwan-Norreel B S.Cryopreservation of apple(Malus domestica Borkh.) shoot tips following encapsulation-dehydration or encapsulation-vitrification[J] Plant Cell Rep,2000,19:768-774.
[3]  Wang Q C,Batuman ,Li P,et al.A simple and efficient cryopreservation of in vitro-grown shoot tips of Troyer''citrange Poncirus trifoliata(L.) Raf.× Citrus sinensis(L.) Osbeck.by encapsulation-vitrification[J] Euphytica,2002,128:135-142.
[4]  Hirai D,Sakai A.Simplified cryopreservation of sweet potato[Ipomoea batatas(L.) Lam.] by optimizing conditions for osmoprotection[J] Plant Cell Rep,2003,21:961-966.
[5]  Moges A D,Shibli R A,Karam N.Cryopreservation of african violet(Saintpaulia Ionantha Wendl.) shoot tips[J] In Vitro Cell Dev-Pl,2004,40:389-395.
[6]  Wang Q C,Mawassi M,Sahar N,et al.Cryopreservation of grapevine(Vitis spp.) embryogenic cell suspensions by encapsulation-vitrification[J] Plant Cell Tissue Organ Cult,2004,77:267-275.
[7]  Wang Q C,Laamanen J,Uosukainen M,et al.Cryopreservation of in vitro-grown shoot tips of raspberry(Rubus idaeus L.) by encapsulation vitrification and encapsulation dehydration[J] Plant Cell Rep,2005,24:280-288.
[8]  李明军,周娜,刘杰,等.怀地黄玻璃化和包埋玻璃化法超低温保存[J] 园艺学报,2008,35:607-610.
[9]  李明军,洪森荣,徐鑫,等.怀山药种质资源的玻璃化法超低温保存[J] 作物学报,2006,32:288-292.
[10]  Li M J,Zhao X T,Hong S R,et al.Cryopreservation of plantlet nodes of Dioscorea opposita Thunb.using a vitrification method[J] CryoLet,2009,30:19-28.
[11]  李海兵,周娜,赵姣,等.怀山药种质资源的包埋玻璃化超低温保存与植株再生[J] 植物学报,2010,45(3):379-383.
[12]  李合生.植物生理生化实验原理和技术[M] 北京:高等教育出版社,2000:134-137.
[13]  Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J] Anal Biochem,1976,72:248-254.
[14]  Arnon D I.Copper enzymes in isolated chloroplasts polyphenoloxidase Beta vulgaris[J] Plant Physiol,1949,24(1):1-15.
[15]  张龙翔,张庭芳,李令媛.生化实验方法与技术[M] 北京:高等教育出版社,1997:161-165.
[16]  何忠效,张树政.电泳[M] 2 版.北京:科学出版社,1999:284-300.
[17]  罗广华,王爱国.植物SOD 的凝胶电泳及活性的显示[J] 植物生理学通讯,1998(6):44-45.
[18]  徐存拴,吉爱玲,夏民,等.用复性电泳技术研究溶酶体蛋白水解酶的性质和活性[J] 河南科学,1998,16(2):185-192.
[19]  Matsumoto T,Sakai A,Takahashi C,et al.Cryopreservation of in vitro-grown apical meristems of wasabi(Wasabia japonica) by encapsulation-vitrification method[J] CryoLet,1995,16:189-196.
[20]  Hirai D,Sakai A.Cryopreservation of in vitro-grown meristems of potato(Solanum tuberosun L.) by encapsulation-vitrification[J] Potato Res,1999,42:153-160.
[21]  伊华林,邓秀新.植物种质离体保存技术研究进展[J] 植物学通报,1999(16):574-581.
[22]  吴雪梅,汤浩茹.包埋玻璃化法超低温保存植物种质的研究进展[J] 植物学通报,2005,22:238-245.
[23]  胡明珏,王君晖.拟南芥悬浮细胞系的玻璃化法超低温保存[J] 细胞生物学杂志,2004,26:81-84.
[24]  Kaity A,Ashmore S E,Drew R A.Field performance evaluation and genetic integrity assessment of cryopreserved papaya clones[J] Plant Cell Rep,2009,28:1421-1430.
[25]  Urbanová M,Kouth J,Cˇ ellárová E.Genetic and biochemical analysis of Hypericum perforatum L.plants regenerated after cryopreservation[J] Plant Cell Rep,2006,25:140-147.
[26]  Liu Y G,Wang X Y,Liu L X.Analysis of genetic variation in surviving apple shoots following cryopreservation by vitrification[J] Plant Sci,2004,166:677-683.
[27]  Dixit-Sharma S,Ahuja-Ghosh S,Mandal B B,et al.Metabolic stability of plants regenerated from cryopreserved shoot tips of Dioscorea deltoidea an endangered medicinal plant[J] Sci Hortic,2005,105:513-517.
[28]  Martín,González-Benito M E.Survival and genetic stability of Dendranthema Grandiflora Tzvelev shoot apices after cryopreservation by vitrification and encapsultiondehydration[J] Cryobiol,2005,51:281-289.
[29]  Ahuja S,Mandal B B,Dixit S,et al.Molecular,phenotypic and biosynthetic stability in Dioscorea floribunda plants derived from cryopreserved shoot tips[J] Plant Sci,2002,163:971-977.
[30]  刘艳霞.菊花茎尖超低温保存及遗传稳定性评价[D] 武汉:华中农业大学,2008:35-36.
[31]  Fabre J,Dereuddre J.Encapsulation-dehydration:a new approach to cryopreservation of Solanum shoot tips[J] CryoLet,1990,11:413-426.
[32]  Scocchi A,Faloci M,Medina R,et al.Plant recovery of cryopreserved apical meristem-tips of Melia azedarach L.using encapsulation/dehydration and assessment of their genetic stability[J] Euphytica,2004,135:29-38.
[33]  Marassi M A,Scocchi A,Gonzalez A M.Plant regeneration from rice anthers cryopreserved by an encapsulation/dehydration technique[J] In Vitro Cell Dev-Pl,2006,42:31-36.
[34]  Paulet F,Engelmann F,Glaszmann J C.Cryopreservation of apices of in vitro plantlets of sugarcane(Saccharum sp.hybrids) using encapsulation/dehydration[J] Plant Cell Rep,1993,12:525-529.
[35]  Martínez D,Tamés R S,Revilla M A.Cryopreservation of in vitro-grown shoot-tips of hop(Humulus lupulus L.) using encapsulation/dehydration[J] Plant Cell Rep,1999,19:59-63.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133