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蓖麻茎秆cDNA-AFLP反应体系的建立

DOI: 10.3969/j.issn.1000-7091.2013.02.016, PP. 86-90

Keywords: cDNA-AFLP,蓖麻,体系建立

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Abstract:

以蓖麻茎秆为试材,研究了影响cDNA-AFLP标记蓖麻差异基因反应体系的几个关键因素,建立了适宜蓖麻的cDNA-AFLP分析体系。结果表明:当裂解液为1.4mL时提取的RNA浓度最高,EcoRⅠ和MseⅠ的组合6h可将ds-cDNA酶切完全,与相应接头连接过夜后产物用于预扩增,预扩增产物稀释15倍时作为选择性扩增模板可以获得条带丰富且重复性好的结果。蓖麻cDNA-AFLP反应体系的建立为进一步研究蓖麻株高、叶型等相关基因奠定了基础。

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