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不同生态区同一基因型烤烟表观遗传的MSAP分析

DOI: 10.3969/j.issn.1000-7091.2013.01.006, PP. 32-36

Keywords: 烤烟,生态区,表观遗传,甲基化,MSAP

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Abstract:

同一烤烟品种在不同的生态烟区具有不同的香型风格,为了研究揭示香型风格形成的机制,以烤烟品种K326为材料,对其在典型清香型和浓香型生态区烟叶基因组DNA进行MSAP分析。结果表明,永州烟草叶片的甲基化水平明显高于玉溪的甲基化水平,其中半甲基化比率高出3.97个百分点,全甲基化比率高出1.74个百分点,MSAP比率高出5.78个百分点,BLAST比对表明,MSAP差异片段与2个烤烟功能基因具有高度同源性。

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