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玉米大斑病菌交配型基因克隆及生物信息学分析

DOI: 10.7668/hbnxb.2014.05.003, PP. 10-15

Keywords: 玉米大斑病菌,交配型,基因克隆,生物信息学分析

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Abstract:

以真菌毒素与分子植物病理学实验室前期分离、鉴定并保存的玉米大斑病菌菌株F1-40(A交配型菌株)、01-23(a交配型菌株)为试验材料,利用候选基因法,通过PCR扩增和GenomeWalking技术,对A交配型菌株和a交配型菌株的MAT基因进行同源片段扩增,从而获得基因全长及其侧翼序列,进一步利用生物信息学方法对扩增得到的MAT基因全长进行保守结构域分析,利用玉米大斑病菌基因组数据库的Blast序列比对软件将MAT1基因和MAT2基因的侧翼序列进行比对。研究结果表明,在不同交配型的玉米大斑病菌中,A交配型的菌株中含有A交配型基因MAT1,a交配型菌株中含有a交配型基因MAT2。2个基因均含有1个内含子,其中MAT1基因编码包括1个完整的MAT-α结合域,该结合域属于MATalpha1家族。MAT2基因编码包含1个完整的HMG-box结合域,该结合域属于DNA结合蛋白中HMG-box家族的Ⅰ类成员,由3个螺旋结构组成,位于133~202个氨基酸残基之间,整体呈U型,结构比较松散,通过高度序列特异性与DNA的小沟结合,参与DNA的复制、转录、翻译等一系列的过程,从而参与玉米大斑病菌的有性生殖过程。MAT1基因和MAT2基因侧翼序列的相似性高于93%。

References

[1]  Debuchy R,Turgeon B G.The mycota I.Growth,differentiation,and sexuality[M].Germany,Berlin:Springer-Verlag,2006:293-323.
[2]  Hicks J,Strathern J N,Klar A J.Transposable mating type genes in Saccharomyces cerevisiae [J].Nature,1979,282(5378):473-478.
[3]  Guzman D,Garber R C,Yoder O C.Cytology of meiosis I and chromosome number of Cochliobolus heterostrophus(Ascomycetes)[J].Canadian Journal of Botany,1982,60:1138-1141.
[4]  Mandel M A,Barker B M,Kroken S,et al.Genomic and population analyses of the mating type loci in Coccidioides species reveal evidence for sexual reproduction and gene acquisition[J].Eukaryotic Cell,2007,6(7):1189-1199.
[5]  Turgeon B G,Bohlmann H,Ciuffetti L M,et al.Cloning and analysis of the mating type genes from Cochliobolus heterostrophus [J].Molecular and General Genetics,1993,238(1/2):270-284.
[6]  Barve M P,Arie T,Salimath S S,et al.Cloning and characterization of the mating type(MAT) locus from Ascochyta rabiei(Teleomorph: Didymella rabiei) and a MAT phylogeny of legume-associated Ascochyta spp.[J].Fungal Genetic Biology,2003,39(2):151-167.
[7]  McGuire I C,Marra R E,Turgeon B G,et al.Analysis of mating-type genes in the chestnut blight fungus, Cryphonectria parasitica [J].Fungal Genetics and Biology,2001,34(2):131-144.
[8]  Nolting N,Pggeler S.A MADS box protein interacts with a mating-type protein and is required for fruiting body development in the homothallic ascomycete Sordaria macrospora [J].Eukaryotic Cell,2006,5(7):1043-1056.
[9]  Turgeon B G,Sharon A,Wirsel S,et al.Structure and function of mating type genes in Cochliobolus spp.and asexual fungi[J].Canadian Journal of Botany,1995,73(S1):783-788.
[10]  Sharon A,Yamaguchi K,Christiansen S,et al.An asexual fungus has the potential for sexual development[J].Molecular and General Genetics,1996,251(1):60-68.
[11]  Yamada-Inagawa T,Klar A J,Dalgaard J Z. Schizosac-charomyces pombe switches mating type by the synthesis-dependent strand-annealing mechanism[J].Genetics,2007,177(1):255-265.
[12]  Houston P L,Broach J R.The dynamics of homologous pairing during mating type interconversion in budding yeast[J].Public Library of Science Genetics,2006,2(6):e98.
[13]  Valencia-Burton M,Oki M,Johnson J,et al.Different mating-type-regulated genes affect the DNA repair defects of Saccharomyce s RAD51,RAD52 and RAD55 mutants[J].Genetics,2006,174(1):41-55.
[14]  Menkis A,Jacobson D J,Gustafsson T,et al.The mating-type chromosome in the filamentous ascomycete Neurospora tetrasperma represents a model for early evolution of sex chromosomes[J].Public Library of Science Genetics,2008,4(3):e1000030.
[15]  Yun S H,Berbee M L,YODER O C,et al.Evolution of the fungal self-fertile reproductive life style from self-sterile ancestors[J].Proceedings of the National Academy of Sciences of the United States of America,1999,96(10):5592-5597.
[16]  Patrik I,Jennifer H B,Gillian T,et al.Lateral transfer of mating system in Stemphylium[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(32):11390-11395.
[17]  Wik L,Karlsson M,Johannesson H.The evolutionary trajectory of the mating-type(mat) genes in Neurospora relates to reproductive behavior of taxa[J].BMC Evolutionary Biology,2008,8:109.

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