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分子鉴定拟南芥myb26/mailsterile35突变体中表达下调基因At2g47500

DOI: 10.7668/hbnxb.2014.05.013, PP. 71-79

Keywords: At2g47500,ms35/myb26,分子鉴定,GUS表达,超量表达

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Abstract:

利用不可产生花粉的突变体作为研究花药和花粉发育的途径,以证实涉及花粉发育的基因及其在基因网络中的作用。研究结果表明,拟南芥中MYB26/MALESTERILE35(MS35)雄性不育基因会调控药室内壁次生加厚发育,影响其后的花药开裂,并可上调木质素生物合成途径。而At2g47500基因是在芯片分析ms35/myb26突变体中涉及花粉发育表达下调的一个未被鉴定的基因。通过生物信息学分析发现,At2g47500基因是一个与微管发育有关的基因,在整个植株都表达,包括花发育阶段。利用拟南芥基因库筛选该基因中的T-DNA插入突变体,并通过PCR鉴定,得到插入位点在启动子和外显子的纯合突变体。通过表型分析和表达筛选发现,At2g47500突变并未对表现型产生影响,表明T-DNA插入位点在外显子的突变体基因并未在花药中表达;但转录表达分析认为,该基因在花粉里表达,并通过启动子:GUS表达结构得到进一步证实。35S启动子超量表达At2g47500,并未导致其在野生型植物中异位的过量表达;将其转入突变体中,基因表达量恢复,也未有表现差异。说明其对花粉发育的影响不明显。定量PCR分析微管发育其他相关基因,以及花发育中次生壁发育有关基因在突变体中的表达,At2g47500对花粉发育影响不明显。在突变体中,大部分基因也未因其缺失、表达受到明显影响。综上所述,该基因在花药发育中不受ms35/myb26的调控,发挥次要作用,受到主效基因的调控。

References

[1]  Lippman B Z,Zamir D.Heterosis:revisting the magic[J].Trends in Genetics,2007,23(2):60-66.
[2]  Cheng S H,Zhuang J Y,Fan Y Y,et al.Progress in research and development on hybrid rice:A super-domesticate in China[J].Annals of Botany,2007,100(5):959-966.
[3]  Sanders P M,Bui A Q,Weterings K,et al.Anther developmental defects in Arabidopsis thaliana male-sterile mutants[J].Sexual Plant Reproduction,1999,11(6):297-322.
[4]  Scott R J,Spielman M,Dickinson H G.Stamen structure and function[J].The Plant Cell,2004,16(S):S46-S60.
[5]  Ma H.Molecular genetic analyses of microsporogenesis and microgametogenesis in flowering plants [J].Annual Review of Plant Biology,2005,56:393-434.
[6]  Wilson Z A,Zhang D B.From Arabidopsis to rice:pathways in pollen development [J].Journal of Experimental Botany,2009,60:479-492.
[7]  Feng X,Dickinson H G.Cell cell interactions during patterning of the Arabidopsis anther [J].Biochemical Society Transactions,2010,38:571-576.
[8]  Wilson Z A,Song J,Taylor B,et al.The final split:the regulation of anther dehiscence[J].Journal of Experimental Botany,2011,62(5,SI):1633-1649.
[9]  Bowman J L,Drews G N,Meyerowitz E M.Expression of the Arabidposis flord homeotic gene AGAMOUS is restricted to specific cell types late in flower development[J].Plant Cell,1991,3:749-758.
[10]  Hiratsu K,Matsui K,Koyama T,et al.Dominant repression of target genes by chimeric repressors that include the EAR motif,a repression domain,in Arabidopsis[J].Plant Journal,2003,34(5):733-739.
[11]  Hiratsu K,Ohta M.The Superman protein is an active repressor whose carboxy-terminal repression domain is required for the development of normal flowers[J].FEBS Letters,2002,514(2/3):351-354.
[12]  Yoshimi Oshima,Nobutaka Mitsuda,Masaru Nakata,et al.Novel vector systems to accelerate functional analysis of transcription factors using chimeric repressor gene-silencing technology(CRES-T) [J].Plant Biotechnology,2011,28:201-210.
[13]  Wagner A.Robustness against mutations in genetic networks of yeast[J].Nature Genetics,2000,24(4):355-361.
[14]  Hanada K,Sawada Y,Kuromori T,et al.Functional compensation of primary and secondary metabolites by duplicate genes in Arabidopsis thaliana [J].Molecular Biology and Evolution,2010,28(1):377-382.
[15]  Mitsuda N,Seki M,Shinozaki K,et al.The NAC transcription factors NST1 and NST2 of Arabidopsis regulate secondary wall thickenings and are required for anther dehiscence[J].Plant Cell,2005,17:2993-3006.
[16]  Ishida T,Hattori S,Sano R,et al.Arabidopsis TRANSPARENT TESTA GLABRA2 is directly regulated by R2R3 MYB transcription factors and is involved in regulation of GLABRA2 transcription in epidermal differentiation[J].Plant Cell,2007,19:2531-2543.
[17]  Koyama T,Furutani M,Tasaka M,et al.MTCP transcription factors control the morphology of shoot lateral organs via negative regulation of the expression of boundaryspecific genes in Arabidopsis[J].Plant Cell,2007,19:473-484.
[18]  Kunieda T,Mitsuda N,Ohme-Takagi M A,et al.NAC family proteins NARS1/NAC2 and NARS2/NAM in the outer integument regulate embryogenesis in Arabidopsis[J].Plant Cell,2008,20(10):2631-2642.
[19]  Gu Z,Steinmetz L M,Gu X,et al.Role of duplicate genes in genetic robustness against null mutations[J].Nature,2003,421:63-66.
[20]  Conant G C,Wagner A.Duplicate genes and robustness to transient gene knock-downs in Caenorhabditis elegans[J].Proc Biol Sci,2004,271:89-96.
[21]  Liang H,Li W H.Gene essentiality,gene duplicability and protein connectivity in human and mouse[J].Trends in Genetics,2007,23(8):375-378.
[22]  Liao B Y,Zhang J Z.Mouse duplicate genes are as essential as singletons[J].Trends in Genetics,2007,23(8):378-381.
[23]  Hanada K,Kuromori T,Myouga F,et al.Evolutionary persistence of functional compensation by duplicate genes in Arabidopsis [J].Genome Biology and Evolution,2009,1:409-414.
[24]  Yoshimi Oshima,Nobutaka Mitsuda,Masaru Nakata,et al.Novel vector systems to accelerate functional analysis of transcription factors using chimeric repressor gene-silencing technology(CRES-T) [J].Plant Biotechnology,2011,28:201-210.
[25]  方先文,张云辉,张所兵,等.利用基因沉默技术创造抗稻瘟病水稻资源[J].华北农学报,2013,28(5):106-109.
[26]  张 怡,徐克东,杨 松,等.蔊菜病毒诱导基因沉默体系构建[J].华北农学报,2013,28(6):65-70.

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