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FASN基因与牦牛肌内脂肪酸组成的相关性研究

DOI: 10.7668/hbnxb.2014.03.029, PP. 159-163

Keywords: FASN基因,牦牛,黄牛,表达量,脂肪酸

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Abstract:

为研究牦牛肌肉中脂肪酸组成特征及其形成机制,以牦牛和黄牛背最长肌为材料,利用气象色谱-质谱联用仪(GC-MS)进行脂肪酸的定性定量分析,并通过Real-timePCR技术检测FASN基因在牦牛和黄牛背最长肌中mRNA的表达水平,同时分析了其与脂肪酸组成的相关性。结果发现,牦牛背最长肌中单不饱和脂肪酸(MUFA)和多不饱和脂肪酸(PUFA)含量均极显著高于黄牛(P<0.01),而饱和脂肪酸(SFA)显著低于黄牛(P<0.05)。牦牛背最长肌中FASN基因的mRNA表达水平极显著高于黄牛(P<0.01)。牦牛FASN基因mRNA表达量只与牦牛C10:0呈极显著正相关(P<0.01),与黄牛脂肪酸组成无显著的相关性(P>0.05)。

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