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来源黑曲霉WP1的两种植酸酶基因的克隆及序列比较

DOI: 10.7668/hbnxb.2009.02.005, PP. 22-26

Keywords: phyA,phyB1,PCR,序列同源性

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Abstract:

为了获得高产植酸酶菌株,利用PCR技术,从黑曲霉WP1中克隆出2种植酸酶基因phyA和phyB,分别命名为phyA1、phyB1.phyA1长1346bp,成熟肽序列不含内含子,共编码448个氨基酸;phyB1基因长1560bp,基因序列含有3段内含子,共编码460个氨基酸.phyA1和phyB1序列同源性很低,只有21.12%.二者都含有1个植酸酶基因保守序列RHGXRXP;但phyA1含2个HD保守序列,phyB1只含有1个.将黑曲霉WP1植酸酶phyA1和phyB1的基因序列在国际基因库中注册,注册号分别为:DQ60711;DQ836360.

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