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口蹄疫病毒株AF/72结构蛋白VP1克隆及其T细胞表位预测

DOI: 10.7668/hbnxb.2010.05.006, PP. 28-32

Keywords: 口蹄疫病毒,T细胞表位,VP1蛋白

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Abstract:

以AF/72RNA为模板,反转录并扩增目的cDNA,然后与pGEM-Teasy体连接并转化JM109菌株,提取的重组质粒用凝胶电泳、PCR和EcoRI酶切法鉴定.应用BIMAS生物学软件对AF/72株结构蛋白VP1的H-2Dd、H-2Kd、H-2Ld、A20限制性T细胞表位进行预测,并对不同限制性的T细胞表位进行对比.结果表明,口蹄疫AF/72株结构蛋白VP1长639bp,编码213个氨基酸.不同限制性的T细胞表位虽然不尽相同,但与已鉴定的T细胞表位相比较,它们之间也有一些类似的、并且可能是通用型的T细胞表位.本试验通过生物信息学方法预测AF/72结构蛋白VP1的T细胞表位,为进一步试验确定T细胞表位和口蹄疫病毒T细胞表位的研究提供了一种有效的方法.同时为口蹄疫病毒表位疫苗的设计提供有效地理论基础.

References

[1]  Pereira H G.Foot-and-mouth disease[M]//Gibbs E P J.Virus Diseases of Foot Animals.London:Academic Press Inc,1981:333-363.
[2]  Eric B,Carmen M,Ruiz-Jarabo,et al.Foot-and-mouth disease virus lacking the VP1 G-H loop:the mutant spectrum uncovers interactions among antigenic site for fitness gain[J].Virology,2001,288:192-202.
[3]  Collen T,Dimarchi R,Doel T R.A T cell epitope in VP1 of foot-and-mouth disease virus is immunodominant for vaccinated cattle[J].Immunol,1991,146(2):749-755.
[4]  Zamorano P,Wigdorovitz A,Chaher M T,et al.Recognition of B and T cell epitopes by cattle immunized with a synthetic peptide containing the major immunogenic site of VP1 FMDV O1 Campos[J].Virology,1994,201(2):383-387.
[5]  Wang J L,Liu M Q,Han J,et al.A peptide of foot-and-mouth disease virus serotype Asia1 generating a neutralizing antibody response,and an immunostimulatory peptide[J].Vet Microbiol,2007,125:224-231.
[6]  Hongjin Bian,Juergen Hammer.Discovery of promiscuous HLA-II-restricted T cell epitopes with TEPITOPE.Methods.2004,34:468-475.
[7]  陈启伟,王永录,张永光,等.口蹄疫病毒株AF/72 VP2的结构模拟与分析[J].华北农学报,2009,24(4):64-69.
[8]  周建华,丛国正,高闪电,等.口蹄疫OA/58 病毒株VP2 蛋白结构模拟与B 细胞抗原表位的分析[J].华北农学报,2008,23(3):1-4.
[9]  van Lierop M J,Wagenaar J P,van Noort J M et al.Sequences derived from the highly antigenic VP1 region 140 to 160 of foot-and-mouth disease virus do not prime for a bovine T-cell response against intact virus[J].J Virol,1995,69:4511-4514.
[10]  Doel T R.FMD vaccines[J].Virus Res,2003,91(1):81-99.
[11]  Bhasin M,Raghava G P S.A hybrid approach for predicting promiscuous MHC class I restricted T cell epitopes[J].Biosci,2007,32(1):31-42.
[12]  Wang P,Morgan A A,Zhang Q,et al.Automating document classification for the Immune Epitope Database[J].BMC Bioinformatics,2007,8:269.
[13]  Bhasin M,Raghava G P S.Prediction of CTL epitopes using QM,SVM and ANN techniques[J].Vaccine,2004,22:3195-3204.

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