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牛β-酪蛋白座位无启动子基因打靶载体的构建

DOI: 10.7668/hbnxb.2010.04.004, PP. 17-24

Keywords: 牛&beta,酪蛋白,LoxP,无启动子,打靶载体,构建

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Abstract:

将带有新霉素基因(neo)和绿色荧光蛋白基因(GFP)的质粒pEGFP-C1进行了改造,去掉neo基因的启动子(Psv40),在无启动子的neo和完整的GFP基因两侧分别加入一个LoxP位点.然后将带有LoxP位点、筛选标记基因和报告基因的结构装入左右分别为牛β-casein基因1590bp和533bp的同源臂结构中,构建成无启动子基因打靶载体pT-1590-LoxP-GFP-LoxP-533.

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