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牛Src基因三个内含子单核苷酸多态性

DOI: 10.7668/hbnxb.2010.02.019, PP. 99-105

Keywords: ,Src,单核苷酸多态性,优势等位基因

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Abstract:

采用巢式PCR、DNA测序、创造酶切位点法PCR(Createdrestrictionsite-PCR,CRS-PCR)及PCR-RFLP(Poly-merasechainreaction-restrictionfragmentlengthpolymorphismassay)方法,研究中国荷斯坦牛、鲁西黄牛及渤海黑牛的Src基因内含子6,8,9的单核苷酸多态性(SNPs),发现了14062(C/T)、17302(G/A)和18107(T/C)共3个SNPs,均为首次报道的位点。Src基因14062(C/T)、17302(G/A)和18107(T/C)3个位点在中国荷斯坦牛和鲁西黄牛两个群体中的优势等位基因相同,分别为C、G、C,其等位基因频率分别为0.671/0.647、0.583/0.640、0.558/0.577,而渤海黑牛在这三个位点的优势等位基因分别为T、G、C,其等位基因频率分别为0.525,0.913,0.763。经χ2适合性检验,中国荷斯坦牛、鲁西黄牛和渤海黑牛三个群体在14062(C/T)位点均达到Hardy-Weinberg平衡状态(P>0.05);在17302(G/A)位点则均未达到Hardy-Weinberg平衡状态(P<0.05);中国荷斯坦牛和鲁西黄牛在18107(T/C)位点均达到Hardy-Weinberg平衡状态(P>0.05),而渤海黑牛未达到Hardy-Weinberg平衡状态(P<0.05)。中国荷斯坦牛和鲁西黄牛在14062(C/T)和18107(T/C)位点均表现为中度多态(0.25

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