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小麦黄花叶病毒河南驻马店分离物的鉴定与全序列分析

DOI: 10.7668/hbnxb.2010.02.002, PP. 5-11

Keywords: 小麦黄花叶病毒,驻马店分离物,病毒检测,基因组全序列

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Abstract:

采集河南省驻马店市13个不同田块小麦样品,利用RT-PCR、ELISA和WesternBlotting检测技术进行检测,结果表明,该地有小麦黄花叶病毒的存在与分布。对检测为阳性的一个分离物全序列进行cDNA克隆和测序,与已报道WYMV河南潢川、江苏扬州、四川雅安和日本分离物的全序列进行比对,RNA1核苷酸一致性为97.1%~98.4%;RNA2为95.1%~98.4%;氨基酸一致性分别为94.8%~97.8%和94.2%~98.2%。利用软件MEGA4.1对上述五个RNA1和RNA2的全序列分别进行进化树分析,结果表明,驻马店分离物与潢川亲缘关系最近,而与四川雅安亲缘关系最远。

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