全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

外源亚精胺对涝胁迫下玉米幼苗根系无氧呼吸代谢的调控效应

DOI: 10.7668/hbnxb.2015.04.020, PP. 110-116

Keywords: 亚精胺,涝胁迫,玉米,无氧呼吸

Full-Text   Cite this paper   Add to My Lib

Abstract:

为了探讨多胺提高玉米抗涝性的机制,以2个抗涝性不同的玉米品种(郑单958,抗涝性较弱;浚单22,抗涝性较强)为材料,设置正常处理、涝胁迫处理、涝胁迫+亚精胺(1mmol/L)3个处理,分别在处理0,2,4,6d取幼苗根测定相对电解质渗漏率、无氧呼吸酶活性(乳酸脱氢酶(LDH)、丙酮酸脱羧酶(PDC)和乙醇脱氢酶(ADH))、代谢产物(乙醇和乳酸)和亚精胺(Spd)含量,并测定幼苗相对干物质增长速率,研究了亚精胺对涝胁迫下玉米幼苗根系呼吸代谢的调控效应。结果表明:在正常生长情况下,2个玉米品种幼苗根系中无氧呼吸酶活性、代谢产物和亚精胺含量均无明显变化,且在2个品种间无明显差异;涝胁迫处理后,2个玉米品种幼苗根系中无氧呼吸酶活性以及乙醇、乳酸、亚精胺含量增加,且抗性较弱的郑单958的LDH活性和乳酸含量增加幅度大于抗性较强的浚单22,而抗性较强的浚单22的ADH活性和乙醇、亚精胺含量增加幅度大于抗性较弱的郑单958;涝胁迫+亚精胺处理后,2个玉米品种幼苗根系中PDC、ADH活性以及乙醇、Spd含量升高,LDH活性和乳酸含量下降,且抗性比较弱的郑单958的变化幅度大于抗性比较强的浚单22。结果显示,亚精胺与涝胁迫下玉米幼苗根系的呼吸代谢之间存在密切关系,亚精胺一方面可以通过提高玉米幼苗根系中无氧呼吸代谢酶PDC和ADH活性以及增加乙醇和Spd含量减少有害物质乙醛对细胞的伤害;另一方面可以通过降低LDH活性抑制乳酸的过量产生,避免细胞质的过度酸化,降低细胞膜的伤害,从而缓解涝胁迫对玉米幼苗造成的伤害,促进干物质的积累和玉米幼苗的生长。

References

[1]  葛均筑,展 茗,赵 明,等.渍涝胁迫对玉米生理生化的影响研究进展[J].中国农学通报,2012,28(21):7-11.
[2]  汪 天,王素平,郭世荣,等.外源亚精胺对根际低氧胁迫下黄瓜幼苗光合作用的影响[J].应用生态学报,2006,17(9):1609-1612.
[3]  刘盼盼,伍大利,刘 畅,等.淹涝和氮形态对苗期玉米伤流液流量及组分的影响[J].华北农学报,2013,28(3):133-138.
[4]  Christianson J A,Llewellyn D J,Dennis E S, et al. Global gene expression responses to waterlogging in roots and leaves of cotton (Gossypium hirsutum L.) [J].Plant and Cell Physiology,2010,51:21-37.
[5]  任佰朝,张吉旺,李 霞,等.大田淹水对夏玉米叶片衰老特性的影响[J].应用生态学报,2014,25(4):1022-1028.
[6]  Jackson M B,Davies W J,Else M A.Pressure-flow relationships,xylem solutes and root hydraulic conductance in flooded tomato plants [J].Annals of Botany,1996,77:17-24.
[7]  Drew M C.Oxygen deficiency and root metabolism:Injury and acclimation under hypoxia and anoxia[J].Annual Review of Plant Physiology and Plant Molecular Biology,1997,48:223-250.
[8]  Bansal R,Srivastava J P.Antioxidative defense system in pigeonpea roots under waterlogging stress[J].Acta Physiologiae Plantarum,2012,34:515-522.
[9]  胡晓辉,郭世荣,李 璟,等.低氧胁迫对黄瓜幼苗根系无氧呼吸酶和抗氧化酶活性的影响[J].武汉植物学研究,2005,23(4):337-341.
[10]  Jia Y,Sun J,Guo S R, et al. Effect of root-applied spermidine on growth and respiratory metabolism in roots of cucumber (Cucumis sativus) seedlings under hypoxia1[J].Russian Journal of Plant Physiology,2010,57(5):648-655.
[11]  Bai T,Li C Y,Ma F W,et al.Exogenous salicylic acid alleviates growth inhibitionand oxidative stress induced by hypoxia stress in malus robusta read[J].Journal of Plant Growth Regulation,2009,28(4):358-366.
[12]  李 程,裴忠孝,甘林叶,等.光周期对春石斛开花及多胺含量的影响[J].植物生理学报,2014,50(8):1167-1170.
[13]  杜红阳,刘怀攀,李潮海,等.植物体内特殊形态多胺与水分胁迫关系研究进展[J].河南农业科学,2007(12):9-12.
[14]  Gong M,Li Y J,Chen S Z.Abscisic acid-induced thermotolerance in maize seedlings is mediated by calcium and associated with antioxidant systems[J].Journal of Plant Physiology,1998,153(4):488-496.
[15]  Mustroph A,Albrecht G.Tolerance of crop plants to oxygen deficiency stress:Fermentative activity and photosynthetic capacity of entire seedlings under hypoxia and anoxia [J].Physiologiae Plantarum,2003,117:508-520.
[16]  Waters I,Morell S,Greenway H,et al.Effects of anoxia on wheat seedlings.Ⅱ.Influence of O2 supply prior to anoxia on tolerance to anoxia,alcoholic fermentation,and sugar levels [J].Journal of Experimental Botany,1991,42:1437-1447.
[17]  Bergmeger H U.Merthods of enzymatic analysis[M].3rd.Verlag Chemse:Weinhein Press,1983:118-125.
[18]  Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding [J].Analytical Biochemistry,1976,72:248-254.
[19]  Kato-Noguchi H.Evaluation of the importance of lactate for the activation of ethanolic fermentation in lettuce roots in anoxia[J].Physiologiae Plantarum,2000,109:28-33.
[20]  Bergmeger H U.Merthods of enzymatic analysis[M].3rd.Verlag Chemse:Weinhenin Press,1983:598-616.
[21]  Kiriakos K,Maria D,Christakis H,et al.A narrow-pore HPLC method for the identification and quantitation of free,conjugated,and bound polyamines [J].Analytical Biochemistry,1993,214:484-489.
[22]  Trought M C T,Drew M C.Effects of waterlogging on young wheat plants (Triticum aestivum L.) and on soil solutes at different temperatures[J].Plant and Soil,1982,69:311-326.
[23]  Roberts J K,Callis J,Jardetzky O,et al.Cytoplasmic acidosis as a determinant of flooding in tolerance in plants[J].Proceedings of the National Academy of Sciences of the United States of America,1984,81:6029-6033.
[24]  Ismond K P,Dolferus R,De-pauw M,et al.Enhanced low oxygen survival in Arabidopsis through increased metabolic flux in the fermentative pathway[J].Plant Physiology,2003,132:1292-1302.
[25]  Armstrong W,Br ndle R,Jackson M B.Mechanisms of flood tolerance in plants[J].Acta Botanica Neerlandica,1994,43:307-358.
[26]  Guo S R,Nada K,Katoh H,et al.Differences between tomato(Lycopersicon esculentum Mill.) and cucumber (Cucumis sativus L.) in ethanol,lactate and malate metabolisms and cell sap pH of roots under hypoxia[J].Journal of the Japanese Society for Horticultural Science,1999,68(1):152-159.
[27]  康云艳,郭世荣,段九菊,等.24-表油菜素内酯对低氧胁迫下黄瓜根系抗氧化系统及无氧呼吸酶活性的影响[J].植物生理与分子生物学学报,2006,32(5):535-542.
[28]  徐迎仓,王 静,刘 华,等.外源精胺对小麦幼苗抗氧化酶活性的促进作用[J].植物生理学报,2001,27(4):349-352.
[29]  Gupta K,Dey A,Gupta B.Plant polyamines in abiotic stress responses[J].Acta Physiologiae Plantarum,2013,35:2015-2036.
[30]  Liu H P,Dong B H,Zhang Y Y,et al.Relationship between osmotic stress and the levels of free,conjugated and bound polyamines in leaves of wheat seedlings [J].Plant Science,2004,166(5):l261-1267.
[31]  Slathia S,Sharma A,Sikander C P.Influence of exogenously applied epibrassinolide and putrescine on protein content antioxidant enzymes and lipid peroxidation in lycopersicon esculentum under salinity stress[J].American Journal of Plant Science,2012,3(6):714-720.
[32]  Barnawal D,Bharti N,Maji D,et al.1-Aminocyclopropane-1-carboxylic acid (ACC) deaminase-containing rhizobacteria protect Ocimum sanctum plants during waterlogging stress via reduced ethylene generation[J].Plant Physiology and Biochemistry,2012,58(9):227-235.
[33]  程明明,杜红阳,刘怀攀.外源亚精胺对涝胁迫下玉米幼苗根和氧化伤害缓解效应[J].南方农业学学报,2015,46(1):36-41.
[34]  Du H Y,Zhou X G,Yang Q H,et al.Changes in H+-ATPase activity and conjugated polyamine contents in plasma membrane purified from developing wheat embryos under short-time drought stress[J].Plant Growth Regulation,2015,75(1):1-10.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133