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新型G/11家族木聚糖酶的设计及生物学分析

DOI: 10.11830/ISSN.1000-5013.2008.04.0559

Keywords: 木聚糖酶, 调和序列, 三级结构, 理化性质, 合成生物学

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Abstract:

运用Bioedit软件,从已知耐热性能较好的G/11木聚糖酶蛋白序列出发,构建调和序列,获得新型木聚糖酶.首先运用InterProScan得出该序列为G/11家族,再运用MotifScan确定其活性部位,对比G/11木聚糖酶的特征序列确定该酶为木聚糖酶.通过SWISS-MODEL对其三级结构进行预测,了解信号肽长度为27个.运用Protparam Tool对其部分理化性质进行预测,得出其理论等电点为6.82,最适温度为81.72℃,高于调和前的任何一条序列.最后,通过选择宿主菌最优密码子获得其基因序列.

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