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卵巢摘除对四氯化碳所致小鼠急性肝损伤的影响

, PP. 873-875

Keywords: 卵巢摘除,四氯化碳,肝组织学,小鼠

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Abstract:

目的研究卵巢摘除对于四氯化碳(CCl4)所致小鼠急性肝损伤肝细胞结构及功能的影响。方法成年Balb/c小白鼠60只,随机分4组假手术组(CG)6只,单纯卵巢切除组(OEG)6只、假手术CCl4染毒组(WOG-C)24只,卵巢切除CCl4染毒组(OEG-C)24只。假手术CCl4染毒组与卵巢切除CCl4染毒组于术后2周给予0.25%四氯化碳橄榄油,0.1mL?10g-1腹腔注射1次。注射后12、24、48和96h取血进行肝功能测定,取肝脏组织进行石蜡切片HE染色观察,并比较肝实质的损伤面积。结果肝脏丙氨酸转氨酶(ALT)和天门冬氨酸转氨酶(AST)检测显示给予CCl424h时,形态学观察显示卵巢切除染毒组肝脏损伤最为严重,与假手术CCl4染毒组比较,差异有统计学意义(P<0.05)。肝实质的损伤假手术染毒组较卵巢切除染毒组轻,且在96h损伤得到修复,而卵巢切除染毒组96h仍处于严重损伤状态(P<0.01)。结论卵巢摘除可明显加重CCL4所致肝损伤的程度,尤其是CCL4注射24h时。

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