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癫痫对大鼠卵巢和生长卵泡的影响

, PP. 860-863

Keywords: 癫痫点燃模型,生长卵泡,多囊卵巢,颗粒细胞,卵泡膜细胞

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Abstract:

目的探讨癫痫对大鼠卵巢和生长卵泡的影响。方法双极电极植入大鼠左侧杏仁核,给予连续电刺激,建立电点燃的癫痫模型;假手术组,电极植入后不给予电刺激;对照组为正常雌性大鼠。癫痫模型大鼠在点燃20d后于动情间期取双侧卵巢,同期其它两组大鼠也分别于动情间期摘取双侧卵巢,右侧卵巢HE染色后光镜下阅片,通过Image-ProPlus6.0软件对窦前卵泡、窦卵泡、闭锁卵泡及卵泡总数进行分析,左侧卵巢选择窦状卵泡进行透射电镜观察。结果癫痫模型组大鼠卵巢增大,卵巢中窦前卵泡、总卵泡数及闭锁卵泡显著增多(P<0.05),窦状卵泡颗粒层变薄,卵泡膜明显增厚,电镜下显示癫痫组大鼠窦状卵泡中的颗粒细胞凋亡增多,增生的卵泡膜细胞更加富含脂滴。结论癫痫可导致大鼠卵巢发生多囊性改变,生长卵泡凋亡增多,卵泡膜细胞增生分泌增强。

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