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CK19mRNA检测在判断IA2-IB2期宫颈癌 前哨淋巴结微转移中的作用

, PP. 1122-1125

Keywords: CK19mRNA,IA2-IB2期宫颈癌,前哨淋巴结,微转移

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Abstract:

目的检测CK19mRNA在IA2-IB2期宫颈癌前哨淋巴结中的表达,探讨其在判断早期宫颈癌淋巴结微转移中的作用。方法应用荧光定量PCR检测CK19mRNA在IA2-IB2期宫颈鳞癌前哨及非前哨淋巴结中的表达,比较CK19mRNA检测与常规组织病理学检查检出前哨淋巴结微转移的阳性率;分析前哨淋巴结中CK19mRNA的阳性表达与宫颈癌患者临床、病理参数的关系;比较CK19mRNA在前哨与非前哨淋巴结中的阳性表达率。结果30例IA2-IB2期宫颈鳞癌患者共检出前哨淋巴结86枚,荧光定量PCR与常规组织病理学检测前哨淋巴结微转移的阳性率分别为32.6%和12.8%(P?0.05);前哨淋巴结CK19mRNA的阳性表达与宫颈癌患者的临床分期、肿瘤浸润深度、肿瘤直径大小及脉管间隙浸润有关(P0.05);前哨淋巴结中CK19mRNA的阳性表达率明显高于非前哨淋巴结中CK19mRNA的阳性表达率(32.6%和4.5%,P<0.05)。结论与传统组织病理学检查比较,检测CK19mRNA在IA2-IB2期宫颈癌前哨淋巴结中的表达能显著提高微转移检出率,可能成为准确评估早期宫颈癌患者淋巴结微转移的指标和方法,进而能更恰当地指导术后辅助治疗并改善患者的预后。

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