全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

沙利度胺对大鼠子宫内膜异位症模型血管生成的影响

, PP. 318-321

Keywords: 子宫内膜异位症,沙利度胺,大鼠,微血管密度,血管生成

Full-Text   Cite this paper   Add to My Lib

Abstract:

目的探讨沙利度胺(thalidomide,THD)对大鼠子宫内膜异位移植物生长和血管生成的影响。方法采用自体移植法建立SD大鼠子宫内膜异位症模型,3周后行剖腹术测量移植物的大小,然后将大鼠随机分为模型对照组(每天腹腔注射生理盐水2mL),孕三烯酮(YSXT)组(腹腔注射孕三烯酮0.5mg?kg-1?d-1),沙利度胺3个剂量组(分别腹腔注射5、20、40mg?kg-1?d-1),联合用药组(腹腔注射孕三烯酮0.5mg?kg-1?d-1,沙利度胺20mg?kg-1?d-1)。4周后再次剖腹评价子宫内膜异位病灶大小和形态学的变化,并通过Ⅷ因子标记异位子宫内膜血管,采用免疫组化法检测异位内膜组织中微血管密度(Microvesseldensity,MVD)。结果各治疗组异位内膜呈现不同程度萎缩,腺体明显减少,移植物体积均小于模型对照组(P<0.05);各治疗组MVD均低于模型对照组(P<0.05),其中以沙利度胺40mg?kg-1?d-1剂量组最为明显。结论沙利度胺对大鼠子宫内膜异位病灶有抑制作用,可能是通过抑制血管生成起作用。

References

[1]  Taylor RN,Yu J,Torres PB,et al.Mechanistic and therapeutic implications of angiogenesis in endometriosis[J].Reprod Sci,2009,16(2):140-146.
[2]  Giannopoulos K,Schmitt M,Wlasiuk P,et al.The high frequency of T regulatory cells in patients with B-cell chronic lymphocytic leukemia is diminished through treatment with thalidomide[J].Leukemia.,2008,22(1):222-224.
[3]  Yang Y,Ge JP,Zhou ZT.Effects of thalidomide on DMBA-induced oral carcinogenesis in hamster with respect to angiogenesis[J].J Oral Pathol Med,2009,38(5):455-462.
[4]  Yagyu T,Kobayashi H,Matsuzaki H,et al.Thalidomide inhibits tumor necrosis factor-alpha-induced interleukin-8 expression in endometriotic stromal cells,possibly through suppression of nuclear factor-kappaB activation[J].J Clin Endocrinol Metab, 2005,90(5):3017-3021.
[5]  Machado DE,Abrao MS,Berardo PT,et al.Vascular density and distribution of vascular endothelial growth factor(VEGF)and its receptor VEGFR-2(Flk-1)are significantly higher in patients with deeply infiltrating endometriosis affecting the rectum[J].Fertil Steril, 2008,90(1):148-155.
[6]  Laschke MW,Menger MD.In vitro and in vivo approaches to study angiogenesis in the pathophysiology and therapy of endometriosis[J].Hum Reprod Update, 2007,13(4):331-342.
[7]  Bourlev V,Volkov N,Pavlovitch S,et al.The relationship between microvessel density,proliferative activity and expression of vascular endothelial growth factor-A and its receptors in eutopic endometrium and endometriotic lesions[J].Reproduction, 2006,132(3):501-509.
[8]  蒋红清.血管生成抑制剂治疗子宫内膜异位症的实验研究[J].中国实用妇科与产科杂志,2007,23(05):361-364.
[9]  Scarpellini F,Sbracia M,Lecchini S,et al.Anti-angiogenesis treatment with thalidomide in endometriosis:A pilot study.In:58th Annual Meeting of the American-Society-for-Reproductive-Medicine[M].2002 Oct 12-17.Seattle,Washington:2002:O228(Abstract).
[10]  王建军,李怀芳,赵平.沙利度胺对子宫内膜异位症的内膜间质细胞体外表达VEGF的影响[J].中国新药与临床杂志,2007,26(9):641-645.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133