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胶原盘状结构域受体2基因缺失对小鼠卵巢功能的影响

Keywords: 细胞外基质,胶原盘状结构域受体2,黄体,不孕

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Abstract:

目的利用胶原盘状结构域受体2(Discoidindomainreceptor2,DDR2)基因缺失小鼠,研究DDR2缺失对卵巢功能的影响,探索DDR2缺失小鼠不孕的原因。方法①阴道脱落细胞涂片结合改良巴氏染色法检测DDR2基因缺失纯合子(SLIE)与野生型(WT)小鼠动情周期的差异。②PMSG+HCG促排卵后在体式显微镜下计数排卵数量差异,HE染色法观察SLIE小鼠与WT小鼠促排卵后卵巢与子宫形态及结构的差异。结果①成年SLIE小鼠缺乏明显的动情期,绝大多数处于动情间期,缺乏规律的动情周期。②促排卵实验发现,SLIE小鼠促排卵数量显著减少;③排卵后黄体生成早期,SLIE小鼠子宫壁薄,体积小;卵巢切片中没有黄体生成;子宫切片HE染色显示子宫内膜薄,腺体及间质增生不明显,未见分泌粘液,未能进入分泌期。结论DDR2缺失导致卵巢对促性腺激素的反应能力降低,促排卵后黄体生成障碍。

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