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PTEN基因过表达对前列腺癌PC-3细胞增殖的影响

Keywords: PTEN,过表达载体,前列腺癌,PC-3细胞

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Abstract:

目的构建PTEN基因的表达载体并转染前列腺癌PC-3细胞,探讨其对前列腺癌PC-3细胞增殖的调节作用。方法设计合成针对PTENPCR引物序列,构建含有PTEN基因的过表达载体。应用RealTimePCR及Westernblot检测重组质粒刺激PC-3细胞后PTEN的mRNA和蛋白的表达水平,应用MTT法检测过表达载体对PC-3细胞增殖的调节作用。结果经酶切、GFP表达证实,成功构建pSicoR-PTEN载体;RealTimePCR及Westernblot结果显示pSicoR-PTEN载体可在PC-3细胞内成功表达PTEN,并且mRNA和蛋白的表达量相对于空质粒组和PC-3对照组显著上升(P<0.05);MTT结果显示PTEN的过表达对PC-3细胞增殖有明显的抑制作用(P<0.05)。结论成功构建的PTEN过表达载体可以有效的提高PC-3细胞内PTEN的mRNA和蛋白表达水平,并可抑制PC-3细胞的增殖。

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