全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

退变颈椎间盘组织中细胞凋亡及TRAIL表达的研究

, PP. 783-785

Keywords: 颈椎间盘,退行性变,细胞凋亡,TRAIL

Full-Text   Cite this paper   Add to My Lib

Abstract:

目的探讨肿瘤坏死因子相关凋亡诱导配体(tumornecroticfactor-relatedapoptosis-inducingligand,TRAIL)在颈椎间盘退变中的作用。方法采用原位DNA片段末端标记(TUNEL)法和免疫组织化学方法,对比观察30例颈椎病退变颈椎间盘组织细胞及30例创伤对照颈椎间盘组织细胞凋亡状态及TRAIL的表达。结果TUNEL染色观察示颈椎病组的髓核内的平均凋亡细胞率为(10.93±1.87)%,高于对照组的(6.43±1.56)%(P<0.01)。SP免疫组织化学染色示颈椎病组髓核内TRAIL染色阳性细胞率为(49.13±15.66)%,高于对照组的(20.26±2.81)%,差异有统计学意义(P<0.01)。将对照组和颈椎病组全部60例颈椎间盘标本合并后进行Pearson相关分析,颈椎间盘髓核内TRAIL染色阳性细胞率与TUNEL阳性细胞率之间呈正相关,相关系数r=0.921(P<0.01)。结论颈椎病椎间盘组织细胞凋亡数与TRAIL阳性细胞数均明显高于创伤对照组,且凋亡与TRAIL的表达呈正相关。

References

[1]  Gavrieli, Ben-Sasson SA. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation[J]. J Cell Biol, 1992, 119(4):493-501.
[2]  Ishihara H,Kanamori M,Kawaguchi Y,et al.Adjacent segment disease after anterior cervical interbody fusion[J].Spine,2004,4(6):624-628.
[3]  Smale G,Nichols NR,Brady DR,et a1.Evidence for apoptotic cell death in Alzheimer's disease[J].Exp Neuro1,1995,133(2):225-230.
[4]  陈凤华,陈翠真,宋旭东.老年性白内障晶状体上皮细胞凋亡的DNA片断分析及TUNEL检测[J].眼科研究,1999,17(6):454-456.
[5]  Usami S,Takumi Y,Fujita S,et al.Cell death in the inner ear associated with aging is apoptosis? [J]. Brain Res,1997,747(1):147-150.
[6]  Blanco FJ,Guitian R,Vazquez-Martul E,et al.Osteoarthritis chondrocytes die by apoptosis.A possible pathway for osteoarthritis pathology[J]. Arthritis Rheum,1998,41(2):284-289.
[7]  Gruber HE Hanley,EN Jr.Analysis of aging and degeneration of the human intervertebal disc comparision of surgical specimens with normal controls[J]. Spine,1998,23(7):751-757
[8]  Roberts S, Johnson WE. Analysis of aging and degeneration of the human intervertebral disc[J]. Spine, 1999, 24(5): 500.
[9]  Zhang L, Niu T, Yang SY, et al.The occurrence and regional distribution of DR4 on herniated disc cells: a potential apoptosis pathway in lumbar intervertebral disc[J]. Spine, 2008, 33(4):422-427.
[10]  牛涛, 陈伯华, 胡有谷.TRAIL 在正常椎间盘细胞中的分布及表达[J].青岛大学医学院学报, 2007, 439(3): 265-266.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133