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11q23异常小儿急性淋巴细胞白血病细胞 对TRAIL的敏感性研究

, PP. 777-782

Keywords: 11q23,急性淋巴细胞白血病,凋亡诱导配体

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Abstract:

目的观察11q23异常小儿急性淋巴细胞白血病(ALL)细胞对肿瘤坏死因子(TNF)相关的凋亡诱导配体(TRAIL)所介导的细胞毒的敏感性,并探讨其机制。方法以11q23异常ALL细胞株3株及原代细胞为研究对象,通过测定细胞表面TRAIL受体表达、TRAIL作用后细胞的存活率及凋亡,观察11q23异常ALL对TRAIL的敏感性。结果除KOCL33对TRAIL部分敏感外,KOCL69、KOPB26及原代细胞对TRAIL高度耐受。原代细胞及3个细胞株DR4均呈阴性,DR5在KOCL33呈强阳性,而在其他细胞株呈阴性,原代细胞及3个细胞株均未表达DcR1及DcR2。结论11q23异常ALL细胞对TRAIL耐受的原因可能是细胞表面凋亡受体表达低下,并有可能是小儿11q23异常ALL迅速克隆扩增及GVL效应差的重要机制之一。

References

[1]  Biondi A, Cimino G, Pieters R, et al. Biological and therapeutic aspects of infant leukemia [J]. Blood, 2000, 96: 24-33.
[2]  何亚香, 薛永权, 何军,等. 11q23异常儿童急性白血病的临床及实验室分析 [J].中华血液学杂志, 2003, 24(7): 358-361.
[3]  Pui CH, Gaynon PS, Boyett JM, et al. Outcome of treatment in childhood acute lymphoblastic leukemia with rearrangements of the 11q23 chromosomal region [J]. Lancet, 2002,359(9321): 1909-1915.
[4]  Reiman JM, Kmieciak M, Manjili MH, et al. Tumor immunoediting and immunosculpting pathways to cancer progression [J]. Semin Cancer Biol, 2007, 17(4): 275-287.
[5]  Takeda K, Hayakawa Y, et al. Involvement of tumor necrosis factor-related apoptosis -inducing ligand in surveillance of tumor metastasis by liver natural killer cells [J]. Nat Med,2001,7: 94-100.
[6]  Walczak H, Miller RE, Ariail K, et al. Tumoricidal activity of tumor necrosis factor-related apoptosis-inducing ligand in vivo [J]. Nat Med,1999,5(2):157-163.
[7]  姚悦萍,欧阳健.重组人可溶性TRAIL分子诱导白血病细胞株凋亡的研究[J].现代免疫学, 2006, 26(3): 234-238.
[8]  Riccioni R, Pasquini L, Mariani G, et al. TRAIL decoy receptors mediate resistance of acute myeloid leukemia cells to TRAIL [J]. Haematologica, 2005, 90: 612-624.
[9]  Smyth MJ, Takeda K, Hayakawa Y, et al. Nature's TRAIL--on a path to cancer immunotherapy [J]. Immunity, 2003, 18: 1-6.
[10]  Cretney E, Takeda K, Yagita H, et al. Increased susceptibility to tumor initiation and metastasis in TNF-related apoptosis-inducing ligand-deficient mice[J]. J Immunol, 2002, 168: 1356-1361.
[11]  Takeda K, Smyth MJ, Cretney E, et al. Critical role for tumor necrosis factor-related apoptosis-inducing ligand in immune surveillance against tumor development [J]. J Exp Med,2002, 195: 161-169.
[12]  Schmaltz C, Alpdogan O, Kappel BJ, et al. T cells require TRAIL for optimal graft-versus-tumor activity[J]. Nat Med, 2002(8): 1433-1437.
[13]  Taketani T, Taki T, Sugita K, et al. FLT3 mutations in the activation loop of tyrosinekinase domain are frequently found in infant ALL with MLL rearrangements and pediatric ALL with hyperdiploidy [J]. Blood,2004, 103: 1085-1088.
[14]  Kolb HJ, Schmid C, Barrett AJ, et al. Graft-versus-leukemia reactions in allogeneic chimeras [J]. Blood, 2003,103: 767-776.
[15]  Pan G, O'Rourke K, Chinnaiyan AM, et al. The receptor for the cytotoxic ligand TRAIL [J].Science, 1997,276: 111-113.
[16]  Pan G, Ni J, Wei YF, et al. An antagonist decoy receptor and death domain-containing receptor for TRAIL [J]. Science, 1997, 277:815-821.

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