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纳洛酮对少突胶质细胞的保护作用

, PP. 654-656

Keywords: 纳洛酮,少突胶质细胞,小胶质细胞,LPS

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Abstract:

目的研究纳洛酮(naloxone)对脂多糖(lipopolysacchar,LPS)激活的小胶质细胞诱导少突胶质细胞损伤的影响。方法共培养大鼠小胶质细胞和少突胶质细胞,用LPS(1μg?mL-1)刺激小胶质细胞,随后用0.1μM纳洛酮进行干预,应用免疫组化方法观察计数少突胶质细胞。结果仅用0.1μM纳洛酮处理细胞,细胞活性基本保持不变,而当小胶质细胞被LPS激活后,与之共培养的大量的少突胶质细胞死亡(P<0.01);小胶质细胞被LPS激活,给予0.1μM纳洛酮处理后,少突胶质细胞的活细胞数显著升高(P<0.05)。结论纳洛酮能够抑制LPS激活的小胶质细胞引起的少突胶质细胞的损伤。

References

[1]  苏静, 陈涵强, 王玮. 少突胶质细胞的特性及与新生儿相关疾病的研究[J]. 海峡预防医学杂志, 2008, 3(14): 101-104.
[2]  孟庆林.盐酸纳洛酮在急诊和急性中毒的应用[J].临床荟萃,1996,11(9):399-420.
[3]  Wong A,Luth HJ,Deuther Conrad W,et a1. Advanced glycation endproducts colocalize with inducible nitric oxide synthase in Alzheimer's disease[J]. Brain Res,2001,920(1-2):32-40.
[4]  Murphy A,Sunohara JR,Sundaram M. Induction of protein kinase C substrates, Myristoylated alanine-rich C kinase substrate(MARCKS)and MARCKS-related protein(MRP), by amyloid β-protein in mouse BV-2 microglial cells[J]. Neurosci Lett,2003,347(1):9-12.
[5]  Gonzalez-Scarano F, Baltuch G. Microglia as mediators of inflammatory and degenerative diseases[J]. Annu Rev Neurosci,1999,22:219-40.
[6]  Li JR, Ramenaden ER, Peng J,et al. Tumor Necrosis Factorαmediates Lipopolysaccharide-induced microglial toxicity to developing oligodendrocytes when astrocytes are present[J]. J Neurosci, 2008,28(20):5321-5330.
[7]  Golde S,Chandran S,Brown GC, et a1. Different pathways for iNOS mediated toxicity in vitro dependent on neuronal maturation and NMDA receptor expression.[J]. J Neurochem,2002,82(2):269-282.
[8]  Merrill JE,Ignarro LJ,Sherman MP,et a1.Microglial cell cytotoxicity of oligodendrocytes is mediated through nitric oxide[J].J Immunol,1993,151(4):2132-2141.
[9]  Deng WB, Pleasure J, Pleasure D. Progress in Periventricular Leukomalacia[J]. Arch Neurol,2008, 65(10):1291-1295.
[10]  Back SA, Han BH, Luo NL,et al.Selective vulnerability of late oligodendrocyte progenitors to hypoxia-ischemia[J].J Neurosci, 2002,22(2): 455-463.
[11]  Mancardi G, Hart BA, Capello E, et al. Restricted immune responses lead to CNS demyelination and axonal damage[J]. J Neuroimmunol, 2000,107(2):178-183.
[12]  Behi ME, Dubucquoi S, Lefranc D, et al. New insights into cell responses involved in experimental autoimmune encephalomyelitis and multiple sclerosis[J]. Immunol Lett, 2005,96(1):11-26.
[13]  Liu B, Hong J S. Role of microglia in inflammation-mediated eurodegenerative diseases:mechanisms and strategies for therapeutic interventions[J].J Pharmacol Exp Ther, 2003,304(1):1-7.
[14]  Emerit J, Edeas M, Bricaire F. Neurodegenerative diseases and oxidative stress[J]. Biomed Pharmacother, 2004, 58(1): 39-46.

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