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华西医学  2015 

肿瘤干细胞分离方法研究进展

DOI: 10.7507/1002-0179.20150393, PP. 1361-1364

Keywords: 肿瘤干细胞,干细胞标志物,流式细胞分选,免疫磁珠分选

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Abstract:

肿瘤干细胞是肿瘤治疗新理论,近年来受到广泛关注,也备受争议。该理论认为肿瘤干细胞是肿瘤细胞的来源,是肿瘤发生耐药、复发、转移的根本原因。目前通过悬浮细胞培养法、流式细胞分选法、免疫磁珠分选法等多种方法,已从多种实体瘤细胞系或组织中分离和鉴定出肿瘤干细胞,包括乳腺癌、脑肿瘤、结肠癌、胰腺癌、卵巢癌等,研究其生物学特性、特异性标志物及调控机制,有望为肿瘤治疗提供新的思路和策略。研究肿瘤干细胞,首先要对其分离和培养,现就目前肿瘤干细胞的分离方法研究进展予以综述。

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