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华西医学  2013 

肾移植患者胞内分枝杆菌感染的分子诊断

DOI: 10.7507/1002-0179.20130306, PP. 984-987

Keywords: 胞内分枝杆菌,分子诊断,肾移植,皮肤软组织感染,hsp65基因

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Abstract:

目的 探讨肾移植患者非结核分枝杆菌(NTM)病临床特点及分子诊断。方法 回顾性分析2011年4月1例皮肤软组织NTM感染的肾移植患者的临床特点,并以其病变组织DNA为模板,聚合酶链反应(PCR)扩增hsp65基因和rpoB基因序列,测序比对鉴定其NTM菌种。结合文献复习NTM病及分析分子生物学技术在移植患者NTM感染诊断中的作用。结果 该肾移植患者系皮肤软组织胞内分枝杆菌感染,临床特点与结核病极其相似,难以进行鉴别诊断。PCR扩增、测序的结果显示hsp65产物和rpoB产物序列与胞内分枝杆菌GeneBank中FJ643456.1及CP003324.1序列100%一致。结论 NTM病的临床表现与结核病相似,分子生物学方法鉴定菌种对移植患者胞内分枝杆菌病的诊断有帮助。

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