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华西医学  2012 

电压依赖性钙通道参与大振幅微小兴奋性突触后电流形成的实验研究

, PP. 905-909

Keywords: 辣椒素,电压依赖性钙通道,微小兴奋性突触后电流,大鼠

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Abstract:

目的 观察电压依赖性钙通道是否作用于大鼠脊髓背角胶状质层(SG)神经元大振幅微小兴奋性突触后电流的形成。方法 选用成年雄性Sprague-Dawley(SD)大鼠,2%~3%异氟烷麻醉后,分离其腰骶部的脊髓,然后切片。采用全细胞电压钳技术,玻璃微电极的电阻为4~6MΩ,钳制电压为?70mV,记录胶状质层神经元微小兴奋性突触后电流(mEPSC)电流。将电流信号用Axopatch200来放大并储存于电脑。对照组和用药结束后,持续采样mEPSC电流30s。mEPSC电流的频率和振幅用Clampfit8.1进行分析。结果 钳制电压为?70mV时,所有SG神经元均有自发性的EPSC。辣椒素增加mEPSC发生的频率和波幅。钴离子抑制辣椒素诱导的大振幅mEPSC。钴离子抑制辣椒素诱导的mEPSC的平均振幅,而不抑制其发生频率。结论 电压依赖性钙离子通道参与了辣椒素引起的痛觉形成。

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