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中华按蚊IRE_BP1基因的表达与氯氰菊酯抗性的相关性初探

DOI: 10.13604/j.cnki.46-1064/r.2015.09.02, PP. 1037-1039

Keywords: 中华按蚊,铁离子应答元件结合蛋白1,荧光定量PCR,表达差异

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Abstract:

目的分析铁离子应答元件结合蛋白1基因在不同中华按蚊品系中表达量的差异。方法根据前期二维电泳筛选获得的差异表达肽段序列,设计相应的PCR引物扩增基因片段,并检测中华按蚊抗性品系、敏感品系、现场采集品系铁离子应答元件结合蛋白1基因表达水平的差异。结果扩增获得了中华按蚊铁离子应答元件结合蛋白1的基因片段,经荧光定量PCR证实,该基因在抗性品系蚊体内的表达量是敏感品系的9.42倍,现场品系是敏感品系的6.38倍。结论中华按蚊铁离子应答元件结合蛋白1基因在杀虫剂抗性通路中有一定的作用,可做为新的抗性治理靶标及基因检测指标。

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