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姜黄素对CsA诱导的HK-2细胞内质网应激的影响

DOI: 10.13604/j.cnki.46-1064/r.2015.09.05, PP. 1047-1050

Keywords: 姜黄素,环孢素A,内质网应激,凋亡

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Abstract:

目的探讨姜黄素对CsA诱导的人近端肾小管上皮细胞(HK-2细胞)内质网应激的影响。方法CsA诱导HK-2细胞发生纤维化改变,同时采用不同浓度姜黄素干预,采用MTT法检测细胞增殖情况,流式细胞术检测细胞凋亡情况,westernblot检测ERS标志性蛋白GRP78及相关凋亡蛋白CHOP的表达。结果用6ng/mLCsA刺激HK-2细胞48h后,细胞增殖明显增强,(1.562±0.125)vs(0.937±0.041),PPPPPP<0.05)。结论姜黄素可以逆转CsA诱导的纤维化,其机制可能与姜黄素能够抑制ERS引起的细胞凋亡有关。

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