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体外LPS预处理小胶质细胞炎性细胞因子分泌反应的探讨

DOI: 10.13604/j.cnki.46-1064/r.2015.07.02, PP. 781-785

Keywords: 小胶质细胞,脂多糖,炎性细胞因子,预处理

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Abstract:

目的通过革兰阴性菌LPS(脂多糖,Lipopolyssacride,LPS)预处理体外培养大脑皮质小胶质细胞,模拟轻型细菌感染,尝试建立体外LPS耐受模型,探讨小胶质细胞促炎细胞因子分泌反应的变化情况。方法体外培养与分离小鼠大脑皮质小胶质细胞,免疫荧光法进行纯度鉴定。纯化的小胶质细胞分为未刺激对照组、0.01μg/mLLPS单次刺激组、1μg/mLLPS刺激组、预处理组(先采用0.01μg/mLLPS刺激18h后,再用1μg/mLLPS刺激6、24、48h)。处理后收获各组细胞培养上清,采用ELISA法检测小胶质细胞TNF-α、IL-6分泌水平与变化趋势。结果经过LPS处理的小胶质细胞(0.01μg/mLLPS单次刺激组、1μg/mLLPS单次刺激组与预处理组)TNF-α与IL-6分泌增加,但预处理组在经过1μg/mLLPS再次刺激不同时间点(6h、24h、48h)分泌的总体水平变化不大(P>0.05)。在6h,1μg/mL单次刺激组、预处理组的TNF-α与IL-6水平比对照组水平明显升高,差异均具有统计学意义(PPP结论体外LPS预处理可在小胶质细胞中诱导部分耐受效应,表现为某些关键炎性细胞因子的分泌抑制,但并非是一种全面的抑制,这可能与细胞来源以及信号通路分子的重新调配有关。

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