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自修复混凝土中微生物矿化方解石的形成机理

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Abstract:

分别从矿化产物、pH值、O2、底物4个方面对用于混凝土裂缝自修复的微生物矿化形成方解石机理进行了研究。结果表明混凝土裂缝自修复细菌矿化产物为方解石型CaCO3,矿化过程需要O2参与;细菌生长过程中pH值从7.0逐渐升高到8.3,碱性环境在细菌矿化过程中起重要作用;CaCO3不是由底物在胞外酶作用下直接分解而得,而是需要经过细菌一系列代谢转换,代谢过程中产生CO2,底物既提供矿化所需的Ca2+源,也提供CO32–来源,其他有机营养物质也可提供CO32–。同时,对该菌株的矿化机理进行了分析,结果表明细菌生长繁殖过程中创造碱性环境,产生CO2,细菌细胞表面带负电荷,能够吸附Ca2+并作为成核位点,在碱性环境下CO2与Ca2+反应形成CaCO3晶体。

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